LOCUS       sequence-95649-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector to express JNK KTR mRuby2 under PGK promoter (With
            Blasticidin Resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pLentiPGK Blast DEST JNKKTRmRuby2
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    97..121
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    150..202
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agggggatcc accggttcgt
       61 cgactagtcc agtgtggtgg aattctgcag atatcaacaa gtttgtacaa aaaagcaggc
      121 tttaaaggaa ccaattcagt cgactggatc tcgagctcaa gcttcgaatt ctgcagtcga
      181 cggtaccgcg ggcccgggat ccaccggccg gtcgccacca tgagtaaccc taagatccta
      241 aaacagagca tgaccttgaa cctggccgac ccggtgggca gtctgaagcc gcacctccgc
      301 gccaagcgct cggccggtct caacgaagac gagtcgccat ctaagaagct ggcgtcgccg
      361 gaggtgtcgt caaggctgga gcgcctgacc ctccagtcca gcactagtgt gtctaagggc
      421 gaagagctga tcaaggaaaa tatgcgtatg aaggtggtca tggaaggttc ggtcaacggc
      481 caccaattca aatgcacagg tgaaggagaa ggcaatccgt acatgggaac tcaaaccatg
      541 aggatcaaag tcatcgaggg aggacccctg ccatttgcct ttgacattct tgccacgtcg
      601 ttcatgtatg gcagccgtac ttttatcaag tacccgaaag gcattcctga tttctttaaa
      661 cagtcctttc ctgagggttt tacttgggaa agagttacga gatacgaaga tggtgg
//
