LOCUS       sequence-95651-e         452 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector to express JNK KTR mRuby2 under PGK promoter (With
            Blasticidin Resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pLentiPGK Blast DEST JNKKTRmRuby2
FEATURES             Location/Qualifiers
     source          1..452
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(9..56)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(104..433)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      118..253
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(172..191)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     418..438
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ctcaccttgt cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt
       61 gctgatcaga tccgaaaatg gatatacaag ctcccgggag ctttttgcaa aagcctaggc
      121 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggcagaggcg gcctcggcct
      181 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag
      241 ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca
      301 tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact
      361 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
      421 ctaactgaca cacattccac agaattaatt cc
//
