LOCUS       sequence-95656-e         529 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 nuclease and gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T
  TITLE     Multiplex genome engineering in human cells using all-in-one 
            CRISPR/Cas9 vector system.
  JOURNAL   Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400.
  PUBMED    24954249
REFERENCE   2  (bases 1 to 529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-06-23"; volume: "4:5400. doi"; pages: " 
            10.1038/srep05400"
COMMENT     Sequence Label: pX330S-7
FEATURES             Location/Qualifiers
     source          1..529
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(314..334)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(341..406)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      406..415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agaagctgtc gtccaccttg gccatctcgt tgctgaagat ctcttgcaga tagcagatcc
       61 ggttcttccg tctggtgtat cttcttctgg cggttctctt cagccgggtg gcctcggctg
      121 tttcgccgct gtcgaacagc agggctccga tcaggttctt cttgatgctg tgccggtcgg
      181 tgttgcccag caccttgaat ttcttgctgg gcaccttgta ctcgtcggtg atcacggccc
      241 agcccacaga gttggtgccg atgtccaggc cgatgctgta cttcttgtcg gctgctggga
      301 ctccgtggat accgaccttc cgcttcttct ttggggccat cttatcgtca tcgtctttgt
      361 aatcaatatc atgatccttg tagtctccgt cgtggtcctt atagtccatg gtggcaccgg
      421 tccaacctga aaaaaagtga tttcaggcag gtgctccagg taattaaaca ttaatacccc
      481 accaaccaac catcccttaa acccttacct cttgctcagc taattacag
//
