LOCUS       sequence-95657-e       11615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inserts GFP immediately downstream of the Nanog coding region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11615)
  AUTHORS   Faddah DA, Wang H, Cheng AW, Katz Y, Buganim Y, Jaenisch R
  TITLE     Single-cell analysis reveals that expression of nanog is biallelic 
            and equally variable as that of other pluripotency factors in mouse 
            ESCs.
  JOURNAL   Cell Stem Cell. 2013 Jul 3;13(1):23-9. doi: 
            10.1016/j.stem.2013.04.019.
  PUBMED    23827708
REFERENCE   2  (bases 1 to 11615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.04"; journalName: "Cell Stem Cell"; date: 
            "2013-07-3- 3"; volume: "13"; issue: "1"; pages: "23-9"
COMMENT     Sequence Label: Nanog-2A-GFP
FEATURES             Location/Qualifiers
     source          1..11615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2310..2366
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(2367..2369,2370..2372,2373..3086)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2367..2369,2370..2372,2373..3083)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2412..2433)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2673..2692)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3020..3041
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3117..3150
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3211..3707
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3246..3269)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3640..3659
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3747..4346
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3747..3766)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4243..4263
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    4903..4936
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(8048..8067)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8049..8067)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8074..8091)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8074..8090)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8083..8105)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8231..8659
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8318..8337)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8528..8549
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             9003..9797
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9057..9076)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9667..9686
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(9815..9883,9884..10675)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10033..10052)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10846..11434
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11335..11354
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11588..11605
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttttcctg
      301 caggctctga agacctgcct cttcaaggca gccctgattc ttctaccagt cccaaacaaa
      361 agctctcaag tcctgaggct gacaagggcc ctgaggagga ggagaacaag gtccttgcca
      421 ggaagcagaa gatgcggact gtgttctctc aggcccagct gtgtgcactc aaggacaggt
      481 ttcagaagca gaagtacctc agcctccagc agatgcaaga actctcctcc attctgaacc
      541 tgagctataa gcaggtgggg tccttagaag ttgctgtaat gtaacacaat gagactgact
      601 tgctaatgaa tcgaactaac gtctggacgt ctctctaact atcctatcct cctatcctat
      661 gcgcctgtta aaatcataat tatgtgattg attgattgat tgattatgta taagtacact
      721 atagctgtct tcagatacac cagaagaggg catcagatct cattacggat ggttgtgagc
      781 caccatgtgg ttgctgggat ttgaactcag gaccttcgga agagcagtca gtgctcttaa
      841 ccgctgagcc atctctccag ccccaattat gtgatttata tatttcccag tttgtttcat
      901 tggttacttt atcttctact tcacctccca cacccatccc caaatcaggg tctcactgta
      961 gccctgactg gtctctgtag accaggctgg gagaggttca cctgcctctg tctcctgggt
     1021 gctggaaata aaggtgtgcc tggctttttt cctttctttt cttttttaag attgtttatt
     1081 tttaaattcc tttagttcat tggtgttttg cctgcatgtg tttgtctttg tgagagtatc
     1141 agatctcctg gactagagtt acagacagtc gtgagctgcc atgtggccgc tgagaattga
     1201 acccaggtcc tctggaagag cagccagtgc tcttaacaga agagccattt cccccagcac
     1261 ctgtcttttt caagaaagag tttcacttta caggcctggc tgtcatgttt aaggtcgggc
     1321 tgtagacctg tctgcattct gtatgtctta gcttgttcga atgtcctgtc actctgcagg
     1381 ttaagacctg gtttcaaaac caaaggatga agtgcaagcg gtggcagaaa aaccagtggt
     1441 tgaagactag caatggtctg attcaggtaa caagatgttt tctctgcttt cctttttccc
     1501 cctccatctt accatcttat tttatgtgtc tgtgccgtgt gtgtgtgtgt gtgtgtgtgt
     1561 gtggctgacc tatggctgtt gggtatggat cccagatttc ctgtaagagc agtcagtggc
     1621 tctgcctttg cttcccagtg tcatcctgat ccatctatgt ccattagtcc tgtccctttc
     1681 ccatccctct tcctccacgc actctttctt gtgatctttg gttctttgag gcaggctctc
     1741 tctatgtagc cctggcagtc agagatctgc ctgcctagtc tcaggagtgc tggggttaac
     1801 ggcctgtgcg gccatgctaa tcttttcagt atttctgcag tttacatctt tagagtcccc
     1861 ttttcactca ttgcattaat cacctctttc agaagggctc agcaccagtg gagtatccca
     1921 gcatccattg cagctatccc cagggctatc tggtgaacgc atctggaagc ctttccatgt
     1981 ggggcagcca gacttggacc aacccaactt ggagcagcca gacctggacc aacccaactt
     2041 ggaacaacca gacctggacc aacccaactt ggagcagcca ggcctggacc gctcagtcct
     2101 ggaacggcca gccttggaat gctgctccgc tccataactt cggggaggac tttctgcagc
     2161 cttacgtaca gttgcagcaa aacttctctg ccagtgattt ggaggtgaat ttggaagcca
     2221 ctagggaaag ccatgcgcat tttagcaccc cacaagcctt ggaattattc ctgaactact
     2281 ctgtgactcc accaggtgaa atagctagcg ccactaactt ctccctgttg aaacaagcag
     2341 gggatgtcga agagaatccc gggccaatgg tgagcaaggg cgaggagctg ttcaccgggg
     2401 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     2461 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     2521 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     2581 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     2641 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     2701 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     2761 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     2821 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     2881 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     2941 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     3001 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     3061 tcggcatgga cgagctgtac aagtaagaat tccgatcata ttcaataacc cttaatataa
     3121 cttcgtataa tgtatgctat acgaagttat taggtctgaa gaggagttta cgtccagcca
     3181 agcttaggat ctcgacctcg aaattctacc gggtagggga ggcgcttttc ccaaggcagt
     3241 ctggagcatg cgctttagca gccccgctgg cacttggcgc tacacaagtg gcctctggcc
     3301 tcgcacacat tccacatcca ccggtagcgc caaccggctc cgttctttgg tggccccttc
     3361 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     3421 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     3481 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     3541 gctttctggg ctcagcagct gggaagggtg ggtccggggg cgggctcagg ggcgggctca
     3601 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     3661 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcatccatc
     3721 tagatctcga gcagctgaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     3781 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     3841 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     3901 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     3961 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     4021 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     4081 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     4141 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     4201 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     4261 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     4321 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
     4381 ggagcgcacg accccatgca tcgatgatat cagatccccg ggatgcagaa attgatgatc
     4441 tattaaacaa taaagatgtc cactaaaatg gaagtttttc ctgtcatact ttgttaagaa
     4501 gggtgagaac agagtaccta cattttgaat ggaaggattg gagctacggg ggtgggggtg
     4561 gggtgggatt agataaatgc ctgctcttta ctgaaggctc tttactattg ctttatgata
     4621 atgtttcata gttggatatc ataatttaaa caagcaaaac caaattaagg gccagctcat
     4681 tcctcccact catgatctat agatctatag atctctcgtg ggatcattgt ttttctcttg
     4741 attcccactt tgtggttcta agtactgtgg tttccaaatg tgtcagtttc atagcctgaa
     4801 gaacgagatc agcagcctct gttccacata cacttcattc tcagtattgt tttgccaagt
     4861 tctaattcca tcagaagctg gtcgagatcc ggaaccctta atataacttc gtataatgta
     4921 tgctatacga agttattagg tccctcgaag aggttcacta ggcgcgccga cttacgcaac
     4981 atctgggctt aaagtcaggg caaagccagg ttccttcctt cttccaaata ttttcatatt
     5041 ttttttaaag atttatttat tcattatatg taagtacact gtagctgtct tcagacactc
     5101 cagaagaggg cgtcagatct tgttacgtat ggttgtgagc caccatgtgg ttgctgggat
     5161 ttgaactcct gaccttcgga agagcagtcg ggtgctctta tccactgagc catctcacca
     5221 gcccctggtt tattttttta attattattt gctttttgtt tatcaagaca gggtttctct
     5281 gcatagctct aattgtcttt gaactagctc tgcagaccag cctggccttg aactcagaga
     5341 tctgcccact tatctttgcc tcctgaatgc tgggaccaaa ggtggcatac caccacacct
     5401 ggcatatata ttgtttattt ctatttctat ttttattggt gccagagcaa acctaggact
     5461 tagaacatgc tgggcaccaa ctcaacttct gagctctatt tacaacttgg tgtgttagtg
     5521 tatttgtctt agttctgaat ttgtcctttt tttagtgtta actctaggct ttggagacag
     5581 tgaggtgcat atactctctc cttcccaaga ataagtgctt gaacaccctt acccacgccc
     5641 acccacccat gctagtcttt tttcttagaa gcgtgggtct tggtatacac tgtgtcattt
     5701 tgaggggtga ggtttaaaag tatatacaaa gtataacgat atggtggcta ctctcgagga
     5761 tgagacagaa ggaccaggag tttgagggta gctcagatat gcaataagtt caaggccaac
     5821 ctgtactatg tttaaatagt aagacagcat ctcgataaaa taataaaact aaagtctcaa
     5881 caaaataaaa gctttcacct attaaggtgc ttgcttgtcc ttggagtccc ccaagagtaa
     5941 ctgctatgtt aatatctgta gaaagatgtt tatatttgac tgtaccatga tgaaccgatg
     6001 ccagctggac tagtttaaac aaaataaaac actaatttta cctttagtgg tcttatcttt
     6061 tgtccagtgg ggtcaatata ctttttattt tggtatgtga aggggagggt cttaaccgag
     6121 ggtccaggaa aatgggtttc ttttggctta accggttggg agggttatac cacaacagtt
     6181 ggaatggcct ggtaaccaga gcaggaggcc agccactcac aatgcatcca gtcaagaact
     6241 ggagagcaat gaataagaaa tgaatgtttt agtgtgggta tgggccctga ggaggccaga
     6301 agcattcgat gatcccctgg agatgaagtt acgtgaagcc acctgatcac ctgatgtggc
     6361 aggctgcagg gaaataagct ctagttctct ggaagaacaa atatttaact gggagagtgg
     6421 tttttattcc tgtgaaataa tagaacttgt ggagagagcc cctgaggtca cacacctagt
     6481 gcttctggga aagaggaggg accacagtct cttgtttttg tgtcacactg gagattaatc
     6541 tgggacttcc tggctccaga tggaggctat cttccctgag cataaccccc taacctacgt
     6601 gggggtgggg gaagatgtca agtgttatca caggagactt gagaggctgt gtgggttcag
     6661 aagtgagttt ggggttttgg agacgattta aatttgctga aaagacagaa gatacgtata
     6721 catctggtgt agttgtgtgt acacttaagg agattaagtt aacgtaactc ctgatgcatt
     6781 aattatagat gttatagtag ttgtatacag tagatttagc taaagcatga tacggaaaaa
     6841 cacagaagaa gcaagcgaca cctagtaact gccgtgcgta aatagccata cctgtcaatc
     6901 acatctttaa ctcctgacca ctcacagggg tacccagccc agcccaagta ggttctgtag
     6961 ctgctcctca gctaaggaga gggctgtttt ggcagtgaga aaaattgtcc ctcattgtgc
     7021 cacagaggct aggacacatt cccttgcaaa gcccagacta attccaccta gaattctcct
     7081 cttctgcttt ctgtcctttg gcatcagtgg ggatggctgc tgtgtcagag atgggacatc
     7141 atcacctcca caaaccctga cgaccgttac tgagagacca ctttgcttca actcttagag
     7201 gtttcgttga taagattcta aaatgtgtgg tcttggggat caaatccagg gactcttaga
     7261 ccctgggtaa gtgctctact actgaacaac actcagtcct tggtgtgaga atccgagctt
     7321 tgattttacc ctttttaggg tggaagagat tccaccaatc acccaggagt ggggctagtt
     7381 caagaggcct ccccagctgc agctttcaga ggctttttgc ttcaggctcc caccaaggga
     7441 cctattcaaa tgccttagtc aactgacatc tgagtcccaa gctgttgcag gaccccagct
     7501 gacttcttta tttaacaaat cctccttttt cccctgctcg ggctcctctc cctcctctgt
     7561 ctggcaactg tccgctttct ctttgtagtt ccccaccagg acctgttttc tctccactgt
     7621 caccaggttt tctgccgact gggtttattc acaacagaat taaccttttc ctttacccag
     7681 agaagccagc ccttggaatt caatggcaga gaaggtctgt gcttctgagc ttgtagtttg
     7741 cactgtccaa ttccgaagac ccaggcaaat ttgatccagg gtgctccgtg ttagacctga
     7801 ggaagaaacc aattccaaga ctgcctgatt aaagcaggtt ttacttagag gaacagcagg
     7861 aactggccag cccatgcaca tgagtaaaac aagcttggtt ggattaattt ggccacgatg
     7921 gtttgcgtta gtggtcttcc ttctcccaac tttgggattg acatttgcgg ccggccaaaa
     7981 gggcgaattc tgcagatatc catcacactg gcggccgctc gagcatgcat ctagagggcc
     8041 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga
     8101 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     8161 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     8221 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     8281 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     8341 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     8401 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     8461 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     8521 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     8581 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     8641 caaaaattta acgcgaattt taacaaaatt cagggcgcaa gggctgctaa aggaagcgga
     8701 acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
     8761 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
     8821 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
     8881 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc
     8941 caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc
     9001 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     9061 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     9121 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     9181 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     9241 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     9301 aggatctcct gtcatcccac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     9361 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     9421 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     9481 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     9541 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     9601 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     9661 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     9721 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     9781 ttgacgagtt cttctgaatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     9841 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     9901 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     9961 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    10021 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
    10081 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    10141 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    10201 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    10261 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    10321 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    10381 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    10441 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    10501 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    10561 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    10621 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    10681 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    10741 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    10801 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    10861 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    10921 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    10981 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    11041 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    11101 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    11161 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    11221 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    11281 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    11341 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    11401 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    11461 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    11521 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    11581 cgagcgcagc gagtcagtga gcgaggaagc ggaag
//
