LOCUS       sequence-95658-e       11407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inserts mCherry immediately downstream of the Nanog coding region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11407)
  AUTHORS   Faddah DA, Wang H, Cheng AW, Katz Y, Buganim Y, Jaenisch R
  TITLE     Single-cell analysis reveals that expression of nanog is biallelic 
            and equally variable as that of other pluripotency factors in mouse 
            ESCs.
  JOURNAL   Cell Stem Cell. 2013 Jul 3;13(1):23-9. doi: 
            10.1016/j.stem.2013.04.019.
  PUBMED    23827708
REFERENCE   2  (bases 1 to 11407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.04"; journalName: "Cell Stem Cell"; date: 
            "2013-07-3- 3"; volume: "13"; issue: "1"; pages: "23-9"
COMMENT     Sequence Label: Nanog-2A-mCherry
FEATURES             Location/Qualifiers
     source          1..11407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2310..2366
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2367..3077
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2512..2530)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2780..2799
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    3110..3143
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(3170..3193)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3567..3586
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3648..4451
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4483..4500)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4489..4600
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4688..4721
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7840..7859)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7841..7859)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7866..7883)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7866..7882)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7875..7897)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8023..8451
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8110..8129)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8320..8341
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             8795..9589
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             join(9607..9675,9676..10467)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9825..9844)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10638..11226
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11127..11146
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11380..11397
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttttcctg
      301 caggctctga agacctgcct cttcaaggca gccctgattc ttctaccagt cccaaacaaa
      361 agctctcaag tcctgaggct gacaagggcc ctgaggagga ggagaacaag gtccttgcca
      421 ggaagcagaa gatgcggact gtgttctctc aggcccagct gtgtgcactc aaggacaggt
      481 ttcagaagca gaagtacctc agcctccagc agatgcaaga actctcctcc attctgaacc
      541 tgagctataa gcaggtgggg tccttagaag ttgctgtaat gtaacacaat gagactgact
      601 tgctaatgaa tcgaactaac gtctggacgt ctctctaact atcctatcct cctatcctat
      661 gcgcctgtta aaatcataat tatgtgattg attgattgat tgattatgta taagtacact
      721 atagctgtct tcagatacac cagaagaggg catcagatct cattacggat ggttgtgagc
      781 caccatgtgg ttgctgggat ttgaactcag gaccttcgga agagcagtca gtgctcttaa
      841 ccgctgagcc atctctccag ccccaattat gtgatttata tatttcccag tttgtttcat
      901 tggttacttt atcttctact tcacctccca cacccatccc caaatcaggg tctcactgta
      961 gccctgactg gtctctgtag accaggctgg gagaggttca cctgcctctg tctcctgggt
     1021 gctggaaata aaggtgtgcc tggctttttt cctttctttt cttttttaag attgtttatt
     1081 tttaaattcc tttagttcat tggtgttttg cctgcatgtg tttgtctttg tgagagtatc
     1141 agatctcctg gactagagtt acagacagtc gtgagctgcc atgtggccgc tgagaattga
     1201 acccaggtcc tctggaagag cagccagtgc tcttaacaga agagccattt cccccagcac
     1261 ctgtcttttt caagaaagag tttcacttta caggcctggc tgtcatgttt aaggtcgggc
     1321 tgtagacctg tctgcattct gtatgtctta gcttgttcga atgtcctgtc actctgcagg
     1381 ttaagacctg gtttcaaaac caaaggatga agtgcaagcg gtggcagaaa aaccagtggt
     1441 tgaagactag caatggtctg attcaggtaa caagatgttt tctctgcttt cctttttccc
     1501 cctccatctt accatcttat tttatgtgtc tgtgccgtgt gtgtgtgtgt gtgtgtgtgt
     1561 gtggctgacc tatggctgtt gggtatggat cccagatttc ctgtaagagc agtcagtggc
     1621 tctgcctttg cttcccagtg tcatcctgat ccatctatgt ccattagtcc tgtccctttc
     1681 ccatccctct tcctccacgc actctttctt gtgatctttg gttctttgag gcaggctctc
     1741 tctatgtagc cctggcagtc agagatctgc ctgcctagtc tcaggagtgc tggggttaac
     1801 ggcctgtgcg gccatgctaa tcttttcagt atttctgcag tttacatctt tagagtcccc
     1861 ttttcactca ttgcattaat cacctctttc agaagggctc agcaccagtg gagtatccca
     1921 gcatccattg cagctatccc cagggctatc tggtgaacgc atctggaagc ctttccatgt
     1981 ggggcagcca gacttggacc aacccaactt ggagcagcca gacctggacc aacccaactt
     2041 ggaacaacca gacctggacc aacccaactt ggagcagcca ggcctggacc gctcagtcct
     2101 ggaacggcca gccttggaat gctgctccgc tccataactt cggggaggac tttctgcagc
     2161 cttacgtaca gttgcagcaa aacttctctg ccagtgattt ggaggtgaat ttggaagcca
     2221 ctagggaaag ccatgcgcat tttagcaccc cacaagcctt ggaattattc ctgaactact
     2281 ctgtgactcc accaggtgaa atagctagcg ccactaactt ctccctgttg aaacaagcag
     2341 gggatgtcga agagaatccc gggccaatgg tgagcaaggg cgaggaggat aacatggcca
     2401 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     2461 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     2521 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     2581 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     2641 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     2701 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     2761 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     2821 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     2881 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     2941 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     3001 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     3061 acgagctgta caagtaatta attaaccagt gtgctggaat tcggcttcaa caacttcgta
     3121 taatgtatgc tatacgaagt tatcagtact tttcccaagg cagtctggag catgcgcttt
     3181 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     3241 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     3301 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     3361 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     3421 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     3481 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
     3541 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     3601 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agccaatatg ggatcggcca
     3661 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     3721 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     3781 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     3841 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     3901 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     3961 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     4021 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     4081 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     4141 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     4201 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     4261 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     4321 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     4381 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     4441 agttcttctg aggggatcaa ttctctagag ctcgctgatc agcctcgact gtgccttcta
     4501 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     4561 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     4621 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     4681 gcaggcaaca acttcgtata atgtatgcta tacgaagtta tcagtacaag ccgaattctg
     4741 cagatatcca tcggcgcgcc gacttacgca acatctgggc ttaaagtcag ggcaaagcca
     4801 ggttccttcc ttcttccaaa tattttcata ttttttttaa agatttattt attcattata
     4861 tgtaagtaca ctgtagctgt cttcagacac tccagaagag ggcgtcagat cttgttacgt
     4921 atggttgtga gccaccatgt ggttgctggg atttgaactc ctgaccttcg gaagagcagt
     4981 cgggtgctct tatccactga gccatctcac cagcccctgg tttatttttt taattattat
     5041 ttgctttttg tttatcaaga cagggtttct ctgcatagct ctaattgtct ttgaactagc
     5101 tctgcagacc agcctggcct tgaactcaga gatctgccca cttatctttg cctcctgaat
     5161 gctgggacca aaggtggcat accaccacac ctggcatata tattgtttat ttctatttct
     5221 atttttattg gtgccagagc aaacctagga cttagaacat gctgggcacc aactcaactt
     5281 ctgagctcta tttacaactt ggtgtgttag tgtatttgtc ttagttctga atttgtcctt
     5341 tttttagtgt taactctagg ctttggagac agtgaggtgc atatactctc tccttcccaa
     5401 gaataagtgc ttgaacaccc ttacccacgc ccacccaccc atgctagtct tttttcttag
     5461 aagcgtgggt cttggtatac actgtgtcat tttgaggggt gaggtttaaa agtatataca
     5521 aagtataacg atatggtggc tactctcgag gatgagacag aaggaccagg agtttgaggg
     5581 tagctcagat atgcaataag ttcaaggcca acctgtacta tgtttaaata gtaagacagc
     5641 atctcgataa aataataaaa ctaaagtctc aacaaaataa aagctttcac ctattaaggt
     5701 gcttgcttgt ccttggagtc ccccaagagt aactgctatg ttaatatctg tagaaagatg
     5761 tttatatttg actgtaccat gatgaaccga tgccagctgg actagtttaa acaaaataaa
     5821 acactaattt tacctttagt ggtcttatct tttgtccagt ggggtcaata tactttttat
     5881 tttggtatgt gaaggggagg gtcttaaccg agggtccagg aaaatgggtt tcttttggct
     5941 taaccggttg ggagggttat accacaacag ttggaatggc ctggtaacca gagcaggagg
     6001 ccagccactc acaatgcatc cagtcaagaa ctggagagca atgaataaga aatgaatgtt
     6061 ttagtgtggg tatgggccct gaggaggcca gaagcattcg atgatcccct ggagatgaag
     6121 ttacgtgaag ccacctgatc acctgatgtg gcaggctgca gggaaataag ctctagttct
     6181 ctggaagaac aaatatttaa ctgggagagt ggtttttatt cctgtgaaat aatagaactt
     6241 gtggagagag cccctgaggt cacacaccta gtgcttctgg gaaagaggag ggaccacagt
     6301 ctcttgtttt tgtgtcacac tggagattaa tctgggactt cctggctcca gatggaggct
     6361 atcttccctg agcataaccc cctaacctac gtgggggtgg gggaagatgt caagtgttat
     6421 cacaggagac ttgagaggct gtgtgggttc agaagtgagt ttggggtttt ggagacgatt
     6481 taaatttgct gaaaagacag aagatacgta tacatctggt gtagttgtgt gtacacttaa
     6541 ggagattaag ttaacgtaac tcctgatgca ttaattatag atgttatagt agttgtatac
     6601 agtagattta gctaaagcat gatacggaaa aacacagaag aagcaagcga cacctagtaa
     6661 ctgccgtgcg taaatagcca tacctgtcaa tcacatcttt aactcctgac cactcacagg
     6721 ggtacccagc ccagcccaag taggttctgt agctgctcct cagctaagga gagggctgtt
     6781 ttggcagtga gaaaaattgt ccctcattgt gccacagagg ctaggacaca ttcccttgca
     6841 aagcccagac taattccacc tagaattctc ctcttctgct ttctgtcctt tggcatcagt
     6901 ggggatggct gctgtgtcag agatgggaca tcatcacctc cacaaaccct gacgaccgtt
     6961 actgagagac cactttgctt caactcttag aggtttcgtt gataagattc taaaatgtgt
     7021 ggtcttgggg atcaaatcca gggactctta gaccctgggt aagtgctcta ctactgaaca
     7081 acactcagtc cttggtgtga gaatccgagc tttgatttta ccctttttag ggtggaagag
     7141 attccaccaa tcacccagga gtggggctag ttcaagaggc ctccccagct gcagctttca
     7201 gaggcttttt gcttcaggct cccaccaagg gacctattca aatgccttag tcaactgaca
     7261 tctgagtccc aagctgttgc aggaccccag ctgacttctt tatttaacaa atcctccttt
     7321 ttcccctgct cgggctcctc tccctcctct gtctggcaac tgtccgcttt ctctttgtag
     7381 ttccccacca ggacctgttt tctctccact gtcaccaggt tttctgccga ctgggtttat
     7441 tcacaacaga attaaccttt tcctttaccc agagaagcca gcccttggaa ttcaatggca
     7501 gagaaggtct gtgcttctga gcttgtagtt tgcactgtcc aattccgaag acccaggcaa
     7561 atttgatcca gggtgctccg tgttagacct gaggaagaaa ccaattccaa gactgcctga
     7621 ttaaagcagg ttttacttag aggaacagca ggaactggcc agcccatgca catgagtaaa
     7681 acaagcttgg ttggattaat ttggccacga tggtttgcgt tagtggtctt ccttctccca
     7741 actttgggat tgacatttgc ggccggccaa aagggcgaat tctgcagata tccatcacac
     7801 tggcggccgc tcgagcatgc atctagaggg cccaattcgc cctatagtga gtcgtattac
     7861 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     7921 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     7981 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg
     8041 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     8101 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     8161 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     8221 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     8281 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     8341 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     8401 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     8461 ttcagggcgc aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg
     8521 gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc
     8581 aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt
     8641 atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc
     8701 ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag
     8761 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     8821 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     8881 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     8941 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     9001 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     9061 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatccc accttgctcc
     9121 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     9181 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     9241 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     9301 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     9361 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     9421 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     9481 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     9541 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttgaaaaagg
     9601 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     9661 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     9721 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     9781 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     9841 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     9901 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     9961 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
    10021 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
    10081 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
    10141 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
    10201 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    10261 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    10321 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    10381 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    10441 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    10501 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    10561 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    10621 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    10681 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    10741 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    10801 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    10861 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    10921 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    10981 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    11041 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    11101 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    11161 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    11221 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    11281 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    11341 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    11401 gcggaag
//
