LOCUS       sequence-95660-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ORF encoding JNK KTR mRuby2 flanked by Gateway sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pENTR-JNKKTRmRuby2
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..101
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    130..182
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca
       61 atgctttttt ataatgccaa ctttgtacaa aaaagcaggc tttaaaggaa ccaattcagt
      121 cgactggatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat
      181 ccaccggccg gtcgccacca tgagtaaccc taagatccta aaacagagca tgaccttgaa
      241 cctggccgac ccggtgggca gtctgaagcc gcacctccgc gccaagcgct cggccggtct
      301 caacgaagac gagtcgccat ctaagaagct ggcgtcgccg gaggtgtcgt caaggctgga
      361 gcgcctgacc ctccagtcca gcactagtgt gtctaagggc gaagagctga tcaaggaaaa
      421 tatgcgtatg aaggtggtca tggaaggttc ggtcaacggc caccaattca aatgcacagg
      481 tgaaggagaa ggcaatccgt acatgggaac tcaaaccatg aggatcaaag tcatcgaggg
      541 aggacccctg ccatttgcct ttgacattct tgccacgtcg ttcatgtatg gcagccgtac
      601 ttttatcaag tacccgaaag gcattcctga tttctttaaa cagtcctttc ctgagggttt
      661 tacttgggaa agagttacga gatacgaaga tggtggagtc gtcaccgtca tgcaggacac
      721 cagccttgag gatggctgtc tcgtttacca cgtccaagtc agaggggtaa actttccctc
      781 caatggtccc gtgatgcaga agaagaccaa gggttgggag cctaatacag agatgatgta
      841 tccagca
//
