LOCUS       sequence-95662-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ORF encoding JNK KTR mRuby2 flanked by Gateway sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pENTR-JNKKTRmRuby2
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    172..271
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgttggaatt taatcgcggc ctcgacgttt cccgttgaat atggctcata acaccccttg
       61 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
      121 caatgtaaca tcagagattt tgagacacgg gccagagctg cagctggatg gcaaataatg
      181 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttctt
      241 ataatgccaa ctttgtacaa gaaagctggg tctagatatc tcgagtgcgg ccgcttactt
      301 gtacagctcg tccatcccac caccaagccc ggcgaacttg gcaactgcgt gttcgcgttg
      361 tactacgaac atttcattgt cactttcctc taacctttcc aggcggtgat caacggcatg
      421 gataccgggc atcttgatgt tcccgacggt cttttttgac ctgtaagttg ttacgaaaga
      481 gcaagacaga tggccaccac catcaacttt cagtgccata tgagtgtatc ccctcagacc
      541 accatctgct ggatacatca tctctgtatt aggctcccaa cccttggtct tcttctgcat
      601 cacgggacca ttggagggaa agtttacccc tctgacttgg acgtggtaaa cgagacagcc
      661 atcctcaagg ctggtgtcct gcatgacggt gacgactcca ccatcttcgt atctcgtaac
      721 tctttcccaa gtaaaaccct cag
//
