LOCUS       sequence-95663-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish 
            larva. The plasmid is in Tol2 transposon backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Vladimirov N, Mu Y, Kawashima T, Bennett DV, Yang CT, Looger LL, 
            Keller PJ, Freeman J, Ahrens MB
  TITLE     Light-sheet functional imaging in fictively behaving zebrafish.
  JOURNAL   Nat Methods. 2014 Jul 27. doi: 10.1038/nmeth.3040.
  PUBMED    25068735
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Jul 27. doi: 10.1038/nmeth.3040."
COMMENT     Sequence Label: Tol2-elavl3-GCaMP6s
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(419..606,607..618)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(652..668)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(652..668)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
       61 caaatgtggt atggctgatt atgatcctct agatcagatc tgcgaagata cggccacggg
      121 tgctcttgat cctgtggctg attttggact gtgctgctcg cagctgctga tgaatcacat
      181 acttcctcca ttttcttcca ctgattgact gttataattt ccctaatttc caggtcaagg
      241 tgctgtgcat tgtggtaata gatgtgacat gacgtcactt ccaaaggacc aatgaacatg
      301 tctgaccaat ttcatataat gtgaaaacga ttttcatagg cagaataaat aacatttaaa
      361 ttaaactggg catcagcgca attcaattgg tttggtaata gcaagggaaa atagaatgaa
      421 gtgatctcca aaaaataagt actttttgac tgtaaataaa attgtaagga gtaaaaagta
      481 cttttttttc taaaaaaatg taattaagta aaagtaaaag tattgatttt taattgtact
      541 caagtaaagt aaaaatcccc aaaaataata cttaagtaca gtaatcaagt aaaattactc
      601 aagtacttta cacctctggt tcttgacccc ctaccttcag caagcccagc agatccacta
      661 gttctagagc ggccgccacc gcggtggagc tccagct
//
