LOCUS       sequence-95667-e         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ORF encoding p38 KTR mClover flanked by Gateway sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pENTR-p38KTRClover
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    174..273
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(309..330)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 catgttggaa tttaatcgcg gcctcgacgt ttcccgttga atatggctca taacacccct
       61 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
      121 tgcaatgtaa catcagagat tttgagacac gggccagagc tgcagctgga tggcaaataa
      181 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgataag caatgctttc
      241 ttataatgcc aactttgtac aagaaagctg ggtctagata tctcgagtgc ggccgcttag
      301 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
      361 agggcggact gatggctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
      421 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcaac gttgtggcgg
      481 atcttgaagt tagccttgat gccgttcttc tgcttgtcgg ccgtgatata gacgttgtgg
      541 ctgttgaagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      601 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcg
//
