LOCUS       sequence-9567-ex       10158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Carey BW, Markoulaki S, Hanna J, Saha K, Gao Q, Mitalipova M, 
            Jaenisch R
  TITLE     Reprogramming of murine and human somatic cells using a single 
            polycistronic vector.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
  PUBMED    19109433
REFERENCE   2  (bases 1 to 10158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Jan 6. 106(1):157-62."
COMMENT     Sequence Label: FUW-KM
FEATURES             Location/Qualifiers
     source          1..10158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1686..1919
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2104..2148
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2446..2563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2622..2638
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2680..2713)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2763..3974
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3811..3830
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5479..5544
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     protein_bind    complement(6958..6991)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7047..7635
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7100..7120)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7518..7529)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7638..7654)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8164..8344
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8406..8994)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8486..8505)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9165..9956,9957..10025))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9788..9807
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10026..10130)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2581 cagcagagat ccagtttggt tagtaccggg cccgctctag tcgaggtcga cggtatcgat
     2641 aagctcgctt cacgagattc cagcaggtcg agggacctaa taacttcgta tagcatacat
     2701 tatacgaagt tatattaagg gttccaagct taagcggcct aacccgtgtc ggctccagat
     2761 ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc
     2821 tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac
     2881 agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt
     2941 aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga
     3001 ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg
     3061 atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg
     3121 gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg
     3181 ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc
     3241 gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag
     3301 cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt
     3361 gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc
     3421 ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc
     3481 ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca
     3541 gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg
     3601 tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg
     3661 tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga
     3721 atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt
     3781 cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg
     3841 ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt
     3901 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg
     3961 cttttttgtt agacgaagct tgggctgcag gtcgactcta gagaattcgc catgaggcag
     4021 ccacctggcg agtctgacat ggctgtcagc gacgctctgc tcccgtcctt ctccacgttc
     4081 gcgtccggcc cggcgggaag ggagaagaca ctgcgtccag caggtgcccc gactaaccgt
     4141 tggcgtgagg aactctctca catgaagcga cttcccccac ttcccggccg cccctacgac
     4201 ctggcggcga cggtggccac agacctggag agtggcggag ctggtgcagc ttgcagcagt
     4261 aacaacccgg ccctcctagc ccggagggag accgaggagt tcaacgacct cctggaccta
     4321 gactttatcc tttccaactc gctaacccac caggaatcgg tggccgccac cgtgaccacc
     4381 tcggcgtcag cttcatcctc gtcttcccca gcgagcagcg gccctgccag cgcgccctcc
     4441 acctgcagct tcagctatcc gatccgggcc gggggtgacc cgggcgtggc tgccagcaac
     4501 acaggtggag ggctcctcta cagccgagaa tctgcgccac ctcccacggc ccccttcaac
     4561 ctggcggaca tcaatgacgt gagcccctcg ggcggcttcg tggctgagct cctgcggccg
     4621 gagttggacc cagtatacat tccgccacag cagcctcagc cgccaggtgg cgggctgatg
     4681 ggcaagtttg tgctgaaggc gtctctgacc acccctggca gcgagtacag cagcccttcg
     4741 gtcatcagtg ttagcaaagg aagcccagac ggcagccacc ccgtggtagt ggcgccctac
     4801 agcggtggcc cgccgcgcat gtgccccaag attaagcaag aggcggtccc gtcctgcacg
     4861 gtcagccggt ccctagaggc ccatttgagc gctggacccc agctcagcaa cggccaccgg
     4921 cccaacacac acgacttccc cctggggcgg cagctcccca ccaggactac ccctacactg
     4981 agtcccgagg aactgctgaa cagcagggac tgtcaccctg gcctgcctct tcccccagga
     5041 ttccatcccc atccggggcc caactaccct cctttcctgc cagaccagat gcagtcacaa
     5101 gtcccctctc tccattatca agagctcatg ccaccgggtt cctgcctgcc agaggagccc
     5161 aagccaaaga ggggaagaag gtcgtggccc cggaaaagaa cagccaccca cacttgtgac
     5221 tatgcaggct gtggcaaaac ctataccaag agttctcatc tcaaggcaca cctgcgaact
     5281 cacacaggcg agaaacctta ccactgtgac tgggacggct gtgggtggaa attcgcccgc
     5341 tccgatgaac tgaccaggca ctaccgcaaa cacacagggc accggccctt tcagtgccag
     5401 aagtgcgaca gggccttttc caggtcggac caccttgcct tacacatgaa gaggcacttt
     5461 gctagcggca gcggcccggt gaaacagact ttgaattttg accttctcaa gttggcggga
     5521 gacgtcgagt ccaaccctgg gcccgcatgc ctcgagggcg cgccactgga tttcctttgg
     5581 gcgttggaaa ccccgcagac agccacgacg atgcccctca acgtgaactt caccaacagg
     5641 aactatgacc tcgactacga ctccgtacag ccctatttca tctgcgacga ggaagagaat
     5701 ttctatcacc agcaacagca gagcgagctg cagccgcccg cgcccagtga ggatatctgg
     5761 aagaaattcg agctgcttcc caccccgccc ctgtccccga gccgccgctc cgggctctgc
     5821 tctccatcct atgttgcggt cgctacgtcc ttctccccaa gggaagacga tgacggcggc
     5881 ggtggcaact tctccaccgc cgatcagctg gagatgatga ccgagttact tggaggagac
     5941 atggtgaacc agagcttcat ctgcgatcct gacgacgaga ccttcatcaa gaacatcatc
     6001 atccaggact gtatgtggag cggtttctca gccgctgcca agctggtctc ggagaagctg
     6061 gcctcctacc aggctgcgcg caaagacagc accagcctga gccccgcccg cgggcacagc
     6121 gtctgctcca cctccagcct gtacctgcag gacctcaccg ccgccgcgtc cgagtgcatt
     6181 gacccctcag tggtctttcc ctacccgctc aacgacagca gctcgcccaa atcctgtacc
     6241 tcgtccgatt ccacggcctt ctctccttcc tcggactcgc tgctgtcctc cgagtcctcc
     6301 ccacgggcca gccctgagcc cctagtgctg catgaggaga caccgcccac caccagcagc
     6361 gactctgaag aagagcaaga agatgaggaa gaaattgatg tggtgtctgt ggagaagagg
     6421 caaacccctg ccaagaggtc ggagtcgggc tcatctccat cccgaggcca cagcaaacct
     6481 ccgcacagcc cactggtcct caagaggtgc cacgtctcca ctcaccagca caactacgcc
     6541 gcacccccct ccacaaggaa ggactatcca gctgccaaga gggccaagtt ggacagtggc
     6601 agggtcctga agcagatcag caacaaccgc aagtgctcca gccccaggtc ctcagacacg
     6661 gaggaaaacg acaagaggcg gacacacaac gtcttggaac gtcagaggag gaacgagctg
     6721 aagcgcagct tttttgccct gcgtgaccag atccctgaat tggaaaacaa cgaaaaggcc
     6781 cccaaggtag tgatcctcaa aaaagccacc gcctacatcc tgtccattca agcagacgag
     6841 cacaagctca cctctgaaaa ggacttattg aggaaacgac gagaacagtt gaaacacaaa
     6901 ctcgaacagc ttcgaaactc tggtgcataa ggcgcgccga attcgtcgag ggacctaata
     6961 acttcgtata gcatacatta tacgaagtta tacatgttta agggttccgg ttccactagg
     7021 tacaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     7081 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     7141 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     7201 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     7261 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     7321 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     7381 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     7441 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     7501 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7561 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7621 gggccgcctc cccgcatcga taccgtcgac ctcgatcgag acctagaaaa acatggagca
     7681 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     7741 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     7801 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7861 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     7921 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
     7981 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
     8041 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
     8101 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
     8161 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8221 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8281 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8341 agcagcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8401 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8461 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8521 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8581 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8641 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8701 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8761 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8821 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8881 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8941 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9001 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9061 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9121 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9181 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9241 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9301 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9361 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9421 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9481 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9541 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9601 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9661 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9721 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9781 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9841 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9901 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9961 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10021 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10081 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10141 cgaaaagtgc cacctgac
//
