LOCUS       sequence-95670-e         573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector to express JNK KTR mClover under PGK promoter 
            (With Puromycin Resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pLentiPGK Puro DEST JNKKTRClover
FEATURES             Location/Qualifiers
     source          1..573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(31..50)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     527..547
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc
       61 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
      121 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
      181 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
      241 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
      301 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
      361 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
      421 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
      481 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
      541 tacgagcggc tcggcttcac cgtcaccgcc gac
//
