LOCUS       sequence-95680-e         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak MS2-CNOT7 (SacI 
            mutated)-P2A-EBFP2 4xFF5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK333
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..70)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ccttgctcac cattgggcct gggttctcct ccacgtcgcc ggcctgcttc agcagagaga
       61 agttggtggc gccagatcca actgactgct tgttggcttc ctcttcatat gcattccctg
      121 tgccattctg tacataggat gaaccagaac caaggccata caaatgacca caatatttgg
      181 catcatcaat atgatcttca aagaacattt ctctcatttt gaaaaaggcc attcctgtga
      241 gcaatgaatc agatcctgcc tgatgttgtg gtcctatccg ttccagctct aactgttctg
      301 ccacctcctg taatccacct ttgagatttt tgcagctctt catgaggtac ttcacatcat
      361 aaatgacagg aaaaaacaat cgaaggatct caaagaagtc aagttcttct tcaggcaagt
      421 tagagttggt taggattttg attaagtagc caaagtcgta accgctatga aatgacaacc
      481 atttgacccc ttcacagagg accactccag aagtcataag aagttctgca aagtactggg
      541 tttcaattcc ttcctcctca tgttttttaa actggatacc agatgttgtt agtagctcta
      601 tagagtcctg ggcatacatg tcctccgtca aattaaattt aaaattaaac tgccaagttg
      661 aagttcctgg aggg
//
