LOCUS       sequence-95682-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector to express JNK KTR mClover under PGK promoter 
            (With Puromycin Resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pLentiPGK Puro DEST JNKKTRClover
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    97..121
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(361..382)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agggggatcc accggttcgt
       61 cgactagtcc agtgtggtgg aattctgcag atatcaacaa gtttgtacaa aaaagcaggc
      121 tccaccatga gtaaccctaa gatcctaaaa cagagcatga ccttgaacct ggccgacccg
      181 gtgggcagtc tgaagccgca cctccgcgcc aagcgctcgg ccggtctcaa cgaagacgag
      241 tcgccatcta agaagctggc gtcgccggag gtgtcgtcaa ggctggagcg cctgaccctc
      301 cagtccagca ctagtatggt gagcaagggc gaggagctgt tcaccggggt tgtgcccatc
      361 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtccgcgg cgagggcgag
      421 ggcgatgcca ccaacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      481 gtgccctggc ccaccctcgt gaccaccttc ggctacggcg tggcctgctt cagccgctac
      541 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      601 gagcgcacca tctctttcaa ggacgacggt acctacaaga cccgcgccga ggtgaagttc
      661 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      721 aacatcctgg
//
