LOCUS       sequence-95696-e         492 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI 
            mutated)-P2A-EBFP2-4xFF5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK332
FEATURES             Location/Qualifiers
     source          1..492
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..66)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gctcaccatt gggcctgggt tctcctccac gtcgccggcc tgcttcagca gagagaagtt
       61 ggtggcgcca gatccaactg actgcttgtt ggcttcctct tcatatgcat tccctgtgcc
      121 attctgtaca taggatgaac cagaaccaag gccatacaaa tgaccacaat atttggcatc
      181 atcaatatga tcttcaaaga acatttctct cattttgaaa aaggccattc ctgtgagcaa
      241 tgaatcagat cctgcctgat gttgtggtcc tatccgttcc agctctaact gttctgccac
      301 ctcctgtaat ccacctttga gatttttgca gctcttcatg aggtacttca catcataaat
      361 gacaggaaaa aacaatcgaa ggatctcaaa gaagtcaagt tcttcttcag gcaagttaga
      421 gttggttagg attttgatta agtagccaaa gtcgtaaccg ctatgaaatg acaaccattt
      481 gaccccttca ca
//
