LOCUS       sequence-95703-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak 
            mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK194
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(5..29)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tggcagcctg cttttttgta caaacttgtt ctagacttgg cggactagac tatgtcgtag
       61 tccattcagg ttagccgtag agagttatag gggcccctct caggtagctg aatgatttaa
      121 cactgctagc tagagtagtc atggccataa ctatgatgga agttcctacg gtttcatacc
      181 ttgattctac tgccttgcac agctctgaaa gaatacccac tcggttccag cgtgttgact
      241 cttcatgcaa tgcccttctc ctgtcatcat catgttcatc gtctgctgcc agaggtttgc
      301 caagcttaaa cagccttttt agggggtctg ccacacggca cgctgtgccg gtcacggagt
      361 cacacaaaat aaaccctcca cagaaataag gcgctttctc gcccaccaca gcatctataa
      421 tcttgacttc catattcaac caggtggcgc acctgtctgc cattaatttg tccgatttga
      481 ctcctttcac gatattgtca tctccaatga atgctgcaca tggtgatccg gttagccgtt
      541 ctctcaacac tctgcttgcg attacaatgt taatgactgt gttcacaaac agtgtgagga
      601 acattccaga tttcatcatg gctccgaatt taaatttagt tttagtgggc aaatgtattg
      661 atgaaatttc gccgaaagcc gcctcaatca gcgtcaacag ctctgcgtcc acacctaagt
      721 cttccagaat cattaacgcg g
//
