LOCUS       sequence-95707-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pENTR-p38KTRmCerulean3
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    172..271
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(340..361)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     689..708
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgttggaatt taatcgcggc ctcgacgttt cccgttgaat atggctcata acaccccttg
       61 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
      121 caatgtaaca tcagagattt tgagacacgg gccagagctg cagctggatg gcaaataatg
      181 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttctt
      241 ataatgccaa ctttgtacaa gaaagctggg tctagatatc tcgagtgcgg ccgcttactt
      301 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
      361 gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac tgggtgctca ggtagtggtt
      421 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
      481 ctgcacgctg ccgtcctcga tgttgcagtt caggccgaag ttggccttga tgccgttctt
      541 ctgcttgtcg gcggtgatat agacgttgcc gtggatggcg ttgtactcca gcttgtgccc
      601 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
      661 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaa
//
