LOCUS       sequence-95710-e         635 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This lentiviral vector can be used to assay Cas9 activity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Doench JG, Hartenian E, Graham DB, Tothova Z, Hegde M, Smith I, 
            Sullender M, Ebert BL, Xavier RJ, Root DE
  TITLE     Rational design of highly active sgRNAs for CRISPR-Cas9-mediated 
            gene inactivation.
  JOURNAL   Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026.
  PUBMED    25184501
REFERENCE   2  (bases 1 to 635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026."
COMMENT     Sequence Label: pXPR_011
FEATURES             Location/Qualifiers
     source          1..635
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(36..55)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     532..552
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 cgaatcaccg acctctctcc ccagggggat ccaccatgac cgagtacaag cccaccgtgc
       61 gcctcgccac ccgcgacgac gtgcccaggg ccgtccgcac cctcgccgcc gctttcgccg
      121 actaccccgc cacccgccac accgtcgatc ccgaccgcca catcgagcgg gtcaccgagc
      181 tgcaagaact cttcctcacc cgcgtcgggc tcgacatcgg caaggtgtgg gtcgctgacg
      241 acggcgccgc agtggctgtc tggaccacac ccgagagcgt cgaagctggg gcggtgttcg
      301 ccgagatcgg ccctcgcatg gccgagttga gcggttcccg gctggccgct cagcaacaga
      361 tggaaggcct cctggctcca caccggccca aggagcccgc atggttcctg gccaccgtcg
      421 gcgtctctcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
      481 aggctgccga gcgcgccggg gtgcccgcct tcctggagac ctccgcaccc cgcaacctcc
      541 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt ggaggtgccc gaaggaccac
      601 gcacctggtg catgacccgc aagcccggtg ccctg
//
