LOCUS       sequence-95720-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ORF encoding ERK KTR mClover flanked by Gateway sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pENTR-ERKKTRClover
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..109
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(412..433)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gataagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctc caccatgaag
      121 ggccgaaagc ctcgggacct ggaacttcca cttagcccaa gcctgctggg tggccaggga
      181 cctgaacgga ctccaggatc aggaacaagc tctggtcttc aggcaccggg gccagcgctt
      241 tcgcccagca agcgtagcgg tctcgaggac ccagcgacgc catcgaagaa gccacgtacc
      301 ccatcggtgt cgtcaaggct ggagcgcctg accctccagt ccagctttca attcccatcc
      361 actagtatgg tgagcaaggg cgaggagctg ttcaccgggg ttgtgcccat cctggtcgag
      421 ctggacggcg acgtaaacgg ccacaagttc agcgtccgcg gcgagggcga gggcgatgcc
      481 accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      541 cccaccctcg tgaccacctt cggctacggc gtggcctgct tcagccgcta ccccgaccac
      601 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      661 atctctttca aggacgacgg tacctacaag acccgcgccg aggtgaagtt cgagggcgac
      721 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      781 gggcacaagc tggagtacaa cttcaacagc cacaacgtct atatcacggc cgacaagcag
      841 aagaacggca tcaaggctaa cttcaagatc cgccacaacg ttgaggacgg cagcgtgca
//
