LOCUS       sequence-95722-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak 
            mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre 
            attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK331
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(20..44)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    185..209
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccttgctcac catggtggca gcctgctttt ttgtacaaac ttgttctaga cttggcggac
       61 tagactatgt cgtagtccat tcaggttagc cgtagagagt tataggggcc cctctcaggt
      121 agctgaatga tttaacactg ctagctagag tagtcatggc cataactatg atggaagtcg
      181 cgccaccact ttgtacaaga aagctgggtg gtaactagct ttaattaaag acttcaagcg
      241 gtttaattaa agacttcaag cggtttaatt aaagacttca agcggtttaa ttaaagactt
      301 caagcggggc gcgtcgagtt acacattgat cctagcagaa gcacaggctg cagggtgacg
      361 gtccatcccg ctctcctggg cacaagacat gggcagcgtg ccatcatcct gctcctccac
      421 ctccggcggg aagccatggc taagcttctt ctgaaggcct ttaatcttct ccacaaggct
      481 tccaagctcc tttctcagct ctccctcgtt gattatcgct gccgaagcgc atggcacctc
      541 aatgcccaca gcccttccaa ggtcgttctt gcttttaacg taaatgtacg gcacattctt
      601 ctcctcgcag aggaggggca gatgagcaac gatctcaggc gggtcaacat cctctgcgat
      661 gtaaacgagc tttgccagtc ccctctccac agcctttgtc gtctcgttgg tacctttctt
      721 taccttaccg ctctccctaa ccttctccag c
//
