LOCUS       sequence-95727-e         543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(16) 2xK-turn 
            Kozak EGFP attB2 AscI (truncated E1) 3'UTR Poly A I-SceI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK317
FEATURES             Location/Qualifiers
     source          1..543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(13..37)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctgtacaagt aaacccagct ttcttgtaca aagtggtggc gcgcctatgt tacgtgcaaa
       61 ggtgattgtc accccccgaa agaccatatt gtgacacacc ctcagtatca cgcccaaaca
      121 tttacagccg cggtgtcaaa aaccgcgtgg acgtggttaa catccctgct gggaggatca
      181 gccgtaatta ttataattgg cttggtgctg gctactattg tggccatgta cgtgctgacc
      241 aaccagaaac ataattgaat acagcagcaa ttggcaagct gcttacatag aactcgcggc
      301 gattggcatg ccgccttaaa atttttattt tatttttctt ttcttttccg aatcggattt
      361 tgtttttaat atttcaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaataggg
      421 ataacagggt aattgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      481 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      541 tca
//
