LOCUS       sequence-95733-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak 
            EGFP-PEST ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK313
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(142..166)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(699..718)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagctgtaca agaagcttag ccatggcttc ccgccggagg tggaggagca ggatgatggc
       61 acgctgccca tgtcttgtgc ccaggagagc gggatggacc gtcaccctgc agcctgtgct
      121 tctgctagga tcaatgtgta aacccagctt tcttgtacaa agtggtggcg cgcctatgtt
      181 acgtgcaaag gtgattgtca ccccccgaaa gaccatattg tgacacaccc tcagtatcac
      241 gcccaaacat ttacagccgc ggtgtcaaaa accgcgtgga cgtggttaac atccctgctg
      301 ggaggatcag ccgtaattat tataattggc ttggtgctgg ctactattgt ggccatgtac
      361 gtgctgacca accagaaaca taattgaata cagcagcaat tggcaagctg cttacataga
      421 actcgcggcg attggcatgc cgccttaaaa tttttatttt atttttcttt tcttttccga
      481 atcggatttt gtttttaata tttcaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      541 aaaataggga taacagggta attgagcaaa aggccagcaa aaggccagga accgtaaaaa
      601 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      661 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      721 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccgg
//
