LOCUS       sequence-95734-e         594 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector to express ERK KTR mClover under CMV promoter 
            (With Puromycin Resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pLentiCMV Puro DEST ERKKTRClover
FEATURES             Location/Qualifiers
     source          1..594
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(53..72)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     549..569
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga
       61 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
      121 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
      181 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
      241 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
      301 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
      361 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
      421 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
      481 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
      541 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccga
//
