LOCUS       sequence-95741-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ORF encoding PKA KTR mClover flanked by gateway sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pENTR-PKAKTRClover
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    172..271
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(307..328)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgttggaatt taatcgcggc ctcgacgttt cccgttgaat atggctcata acaccccttg
       61 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
      121 caatgtaaca tcagagattt tgagacacgg gccagagctg cagctggatg gcaaataatg
      181 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttctt
      241 ataatgccaa ctttgtacaa gaaagctggg tctagatatc tcgagtgcgg ccgcttaggc
      301 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
      361 ggcggactga tggctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
      421 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcaacgt tgtggcggat
      481 cttgaagtta gccttgatgc cgttcttctg cttgtcggcc gtgatataga cgttgtggct
      541 gttgaagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
      601 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
      661 gtaggtaccg tcgtccttga aagagatggt gcgctcctgg acgtagcctt cgggcatggc
      721 ggacttga
//
