LOCUS       sequence-95754-e         635 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector to express PKA KTR mClover under PGK promoter 
            (With Puromycin Resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pLentiPGK Puro DEST PKAKTRClover
FEATURES             Location/Qualifiers
     source          1..635
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    99..123
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(159..180)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga
       61 ggttgattgt cgagcggccg ccactgtgct ggatatcaac cactttgtac aagaaagctg
      121 ggtctagata tctcgagtgc ggccgcttag gcggcggtca cgaactccag caggaccatg
      181 tgatcgcgct tctcgttggg gtctttgctc agggcggact gatggctcag gtagtggttg
      241 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
      301 tgcacgctgc cgtcctcaac gttgtggcgg atcttgaagt tagccttgat gccgttcttc
      361 tgcttgtcgg ccgtgatata gacgttgtgg ctgttgaagt tgtactccag cttgtgcccc
      421 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
      481 tcgccctcga acttcacctc ggcgcgggtc ttgtaggtac cgtcgtcctt gaaagagatg
      541 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
      601 tggtcggggt agcggctgaa gcaggccacg ccgta
//
