LOCUS       sequence-95757-e         604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector to express PKA KTR mClover under PGK promoter 
            (With Puromycin Resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pLentiPGK Puro DEST PKAKTRClover
FEATURES             Location/Qualifiers
     source          1..604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    97..121
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(382..403)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agggggatcc accggttcgt
       61 cgactagtcc agtgtggtgg aattctgcag atatcaacaa gtttgtacaa aaaagcaggc
      121 tccaccatgg agatgccaga ggaacccgcc aacagtgggc attcactacc cccggtttat
      181 atttacagtc ccgagtatgt cagcatctgc gactcccttg tgaaggtccc caaacgggcc
      241 agtatggtca acgaagacga ggcgccatct agaaggaagg cgtcgggtca ggtgtcgtca
      301 aggctggagc gcctgaccct ccagtccagc actagtatgg tgagcaaggg cgaggagctg
      361 ttcaccgggg ttgtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      421 agcgtccgcg gcgagggcga gggcgatgcc accaacggca agctgaccct gaagttcatc
      481 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
      541 gtggcctgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      601 atgc
//
