LOCUS       sequence-95772-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed murine IFITM3-C71,72A with an N-terminal HA tag in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Yount JS, Moltedo B, Yang YY, Charron G, Moran TM, Lopez CB, Hang HC
  TITLE     Palmitoylome profiling reveals S-palmitoylation-dependent antiviral 
            activity of IFITM3.
  JOURNAL   Nat Chem Biol. 2010 Aug;6(8):610-4. doi: 10.1038/nchembio.405. Epub 
            2010 Jul 4.
  PUBMED    20601941
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.405"; journalName: "Nat Chem Biol"; date: 
            "2010-08"; volume: "6"; issue: "8"; pages: "610-4"
COMMENT     Sequence Label: pCMV-HA-mIFITM3-C71,72A
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..42
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    520..654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(544..563)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     598..617
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(765..781)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(765..781)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(778..800)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    789..805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(813..819,820..837,838..843))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    858..879
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgcgggccca ccatgtaccc atacgatgtt ccagattacg ctcttatggc catggaggcc
       61 cgaattccca tgaaccacac ttctcaagcc ttcatcaccg ctgccagtgg aggacagccc
      121 ccaaactacg aaagaatcaa ggaagaatat gaggtggctg agatgggggc accgcacgga
      181 tcggcttctg tcagaactac tgtgatcaac atgcccagag aggtgtcggt gcctgaccat
      241 gtggtctggt ccctgttcaa tacactcttc atgaacttcg cagcactggg cttcatagcc
      301 tatgcctact ccgtgaagtc tagggatcgg aagatggtgg gtgatgtgac tggagcccag
      361 gcctacgcct ccactgctaa gtgcctgaac atcagcacct tggtcctcag catcctgatg
      421 gttgttatca ccattgttag tgtcatcatc attgttctta acgctcaaaa ccttcacact
      481 taagtcgacc gagatctctc gaggtaccgc ggccgcgggg atccagacat gataagatac
      541 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
      601 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
      661 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttcggat
      721 cctctagagt cgatctgcag gcatgctagc ttggcgtaat catggtcata gctgtttcct
      781 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      841 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcg
//
