LOCUS       sequence-95780-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses yeast Atg13 under the endogenous promoter with a GFP 
            N-terminal tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  TITLE     GFP-Atg13
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HC080
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..37
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     22..38
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(186..214)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 agctggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcat
       61 aggtaccaaa tacattccac ttacaccttc caggctcaag tcttgaaaag aaagcagaac
      121 atacagcccg gttgaatagc atgagtctgc ctagaatgag taaaggagaa gaacttttca
      181 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
      241 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
      301 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcgcg tatggtcttc
      361 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc
      421 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
      481 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
      541 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
      601 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac
      661 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
      721 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
      781 a
//
