LOCUS       sequence-95795-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed human IFITM3 with an N-terminal HA tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Yount JS, Moltedo B, Yang YY, Charron G, Moran TM, Lopez CB, Hang HC
  TITLE     Palmitoylome profiling reveals S-palmitoylation-dependent antiviral 
            activity of IFITM3.
  JOURNAL   Nat Chem Biol. 2010 Aug;6(8):610-4. doi: 10.1038/nchembio.405. Epub 
            2010 Jul 4.
  PUBMED    20601941
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.405"; journalName: "Nat Chem Biol"; date: 
            "2010-08"; volume: "6"; issue: "8"; pages: "610-4"
COMMENT     Sequence Label: pCMV-HA-hIFITM3
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(476..502)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          566..662
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     complement(779..803)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(800..899)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(829..849)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tgtctggatc cccgcggccg cggtacctcg agagatctcg gtcgacctat ccataggcct
       61 ggaagatcag cactgggatg acgatgagca gaatggtcat gaggatgccc agaatcaggg
      121 cccagatgtt caggcacttg gcggtggagg cataggcctg ggccccggtc acgtcgccaa
      181 ccatcttcct gtccctagac ttcacggagt aggcgaatgc tatgaagccc aggcagcagg
      241 ggttcatgaa gagggtgttg aacagggacc agacgacatg gtcgggcacg gaggtctcgc
      301 tgcggatgtg gatcacggtg gacgtcgggg gagcagggtt gtggggcgcc cccagcacag
      361 ccacctcgtg ctcctccttg agcatctcat agttgggggg ctggccactg ttgacaggag
      421 agaagaaggt ttggacagtg tgattcatgg gaattcgggc ctccatggcc ataagagcgt
      481 aatctggaac atcgtatggg tacatggtgg gcccgcgggt acaattccgc agcttttaga
      541 gcagaagtaa cacttccgta caggcctaga agtaaaggca acatccactg aggagcagtt
      601 ctttgatttg caccaccacc ggatccggga cctgaaataa aagacaaaaa gactaaactt
      661 accagttaac tttctggttt ttcagttcct ctagtaccgg atcctctaga gtccggaggc
      721 tggatcggtc ccggtgtctt ctatggaggt caaaacagcg tggatggcgt ctccaggcga
      781 tctgacggtt cactaaacga gctctgctta tatagacctc ccaccgtaca cgcctaccgc
      841 ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt
      901 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
      961 accgctatcc acgcccattg atgtactgcc aaa
//
