LOCUS       sequence-95796-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses yeast Atg13 under the endogenous promoter with a 3xHA 
            N-terminal tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Cheong H, Nair U, Geng J, Klionsky DJ
  TITLE     The Atg1 kinase complex is involved in the regulation of protein 
            recruitment to initiate sequestering vesicle formation for 
            nonspecific autophagy in Saccharomyces cerevisiae.
  JOURNAL   Mol Biol Cell. 2008 Feb;19(2):668-81. Epub 2007 Dec 12.
  PUBMED    18077553
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2008-02"; volume: "19(2)"; pages: "668-81. Epub 2007 
            Dec 12"
COMMENT     Sequence Label: HC078 (3xHA-Atg13)
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     16..32
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     17..33
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             161..250
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 taccgggtcc ccccctcgag gtcgacggta tcgataagct tgatatcgaa ttcataggta
       61 ccaaatacat tccacttaca ccttccaggc tcaagtcttg aaaagaaagc agaacataca
      121 gcccggttga atagcatgag ctctgcctag atccatcttt tacccatacg atgttcctga
      181 ctatgcgggc tatccctatg acgtcccgga ctatgcaggg tcctatccat atgacgttcc
      241 agattacgct gctcagtgcg gatctagggt tgccgaagag gacatcgaga agcaagtcct
      301 tcaattgata gacagctttt ttctgaagac tacactacta atatgctcca ccgaatcaag
      361 tcgataccag tcttctacag aaaatatatt cctatttgac gacacatggt ttgaagatca
      421 ctcagaatta gtgagtgagc tacccgagat aatatcaaaa tggtctcact acgatggtcg
      481 aaaagagttg ccacccttag tggtagagac atatttggat ttaagacagt taaactcgtc
      541 tcatttagtt agattaaagg accacgaagg ccatttgtgg aacgtttgca aaggaactaa
      601 gaagcaggaa atcgtgatgg aacgttggct tatcgaatta gataattcat ccccaacttt
      661 caaatcatac agtgaagatg agactgatgt taatgaactt tctaaacagc tagtccttct
      721 cttccgttat ttgttgactt taatacagtt actacccaca ac
//
