LOCUS       sequence-95808-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of OleD (DQ195536) under arabinose promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  TITLE     Anderson Lab Glucosyltransferase plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBAD-OleD
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(171..262)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(281..308)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(400..486)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     639..656
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(712..729)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(745..774)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
       61 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
      121 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
      181 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
      241 tgtatttaga aaaataaaca aaagagtttg tagaaacgca aaaaggccat ccgtcaggat
      301 ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
      361 gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
      421 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
      481 tatttgatgc ctggcagttc cctactctcg catggggaga ccccacacta ccatcggcgc
      541 tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc
      601 caggcaaatt ctgttttatc agaccgcttc tgcgttctga tttaatctgt atcaggctga
      661 aaatcttctc tcatccgcca aaacagccaa gctggagacc gtttaaactc aatgatgatg
      721 atgatgatgg tcgacggcgc tattcagatc ctcttctgag atgagttttt gttc
//
