LOCUS       sequence-95813-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of OleD (DQ195536) under T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  TITLE     Anderson Lab Glucosyltransferase plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET-OleD
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    67..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             106..128
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa tacgactcac
       61 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
      121 gaaggagata taccatggct accacccaga ccactcccgc ccacatcgcc atgttctcca
      181 tcgccgccca cggccatgtg aaccccagcc tggaggtgat ccgtgaactc gtcgcccgcg
      241 gccaccgggt cacgtacgcc attccgcccg tcttcgccga caaggtggcc gccaccggcg
      301 cccggcccgt cctctaccac tccaccctgc ccggccccga cgccgacccg gaggcatggg
      361 gaagcaccct gctggacaac gtcgaaccgt tcctgaacga cgcgatccag gcgctcccgc
      421 agctcgccga tgcctacgcc gacgacatcc ccgatctcgt cctgcacgac atcacctcct
      481 acccggcccg cgtcctggcc cgccgctggg gcgtcccggc ggtctccctc tccccgaacc
      541 tcgtcgcctg gaagggttac gaggaggagg tcgccgagcc gatgtggcgc gaaccccggc
      601 agaccgagcg cggacgggcc tactacgccc ggttcgaggc atggctgaag gagaacggga
      661 tcaccgagca cccggacacg ttcgccagtc atccgccgcg ctccctggtg ctcatcccga
      721 aggcgctcca gccgcacgcc gaccgg
//
