LOCUS       sequence-95817-e         545 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of 71B2 (Q9M156) under T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 545)
  TITLE     Anderson Lab Glucosyltransferase plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET-72B1
FEATURES             Location/Qualifiers
     source          1..545
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     75..92
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    107..246
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(172..191)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     332..354
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(348..539)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 tgcattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
       61 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
      121 ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg
      181 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
      241 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
      301 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
      361 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc
      421 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt
      481 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact
      541 gatgc
//
