LOCUS       sequence-95820-e         562 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of 71B2 (Q9M156) under T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 562)
  TITLE     Anderson Lab Glucosyltransferase plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET-72B1
FEATURES             Location/Qualifiers
     source          1..562
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    67..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             106..128
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa tacgactcac
       61 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
      121 gaaggagata taccatggag gaatccaaaa cacctcacgt tgcgatcata ccaagtccgg
      181 gaatgggtca tctcatacca ctcgtcgagt ttgctaaacg actcgtccat cttcacggcc
      241 tcaccgttac cttcgtcatc gccggcgaag gtccaccatc aaaagctcag agaaccgtcc
      301 tcgactctct cccttcttca atctcctccg tctttctccc tcctgttgat ctcaccgatc
      361 tctcttcgtc cactcgcatc gaatctcgga tctccctcac cgtgactcgt tcaaacccgg
      421 agctccggaa agtcttcgac tcgttcgtgg agggaggtcg tttgccaacg gcgctcgtcg
      481 tcgatctctt cggtacggac gctttcgacg tggccgtaga atttcacgtg ccaccgtata
      541 ttttctactc cgatgcggcc aa
//
