LOCUS       sequence-95830-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses murine IFITM3-K104A with an N-terminal HA tag in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Yount JS, Karssemeijer RA, Hang HC
  TITLE     S-palmitoylation and ubiquitination differentially regulate 
            interferon-induced transmembrane protein 3 (IFITM3)-mediated 
            resistance to influenza virus.
  JOURNAL   J Biol Chem. 2012 Jun 1;287(23):19631-41. doi: 
            10.1074/jbc.M112.362095. Epub 2012 Apr 17.
  PUBMED    22511783
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.362095"; journalName: "J Biol Chem"; date: 
            "2012-06-1- 1"; volume: "287"; issue: "23"; pages: "19631-41"
COMMENT     Sequence Label: pCMV-HA-mIFITM3-K104A
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..29
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    507..641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(531..550)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     585..604
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(752..768)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(752..768)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(765..787)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    776..792
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(800..806,807..824,825..830))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    845..866
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgtacccata cgatgttcca gattacgctc ttatggccat ggaggcccga attcccatga
       61 accacacttc tcaagccttc atcaccgctg ccagtggagg acagccccca aactacgaaa
      121 gaatcaagga agaatatgag gtggctgaga tgggggcacc gcacggatcg gcttctgtca
      181 gaactactgt gatcaacatg cccagagagg tgtcggtgcc tgaccatgtg gtctggtccc
      241 tgttcaatac actcttcatg aacttctgct gcctgggctt catagcctat gcctactccg
      301 tgaagtctag ggatcggaag atggtgggtg atgtgactgg agcccaggcc tacgcctcca
      361 ctgctgcgtg cctgaacatc agcaccttgg tcctcagcat cctgatggtt gttatcacca
      421 ttgttagtgt catcatcatt gttcttaacg ctcaaaacct tcacacttaa gtcgaccgag
      481 atctctcgag gtaccgcggc cgcggggatc cagacatgat aagatacatt gatgagtttg
      541 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
      601 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
      661 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttcggatcct ctagagtcga
      721 tctgcaggca tgctagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      781 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      841 cctaatgagt gagctaactc acattaattg
//
