LOCUS       sequence-95841-e         592 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a granzyme B FRET reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Choi PJ, Mitchison TJ
  TITLE     Imaging burst kinetics and spatial coordination during serial 
            killing by single natural killer cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6488-93. doi: 
            10.1073/pnas.1221312110. Epub 2013 Apr 1.
  PUBMED    23576740
REFERENCE   2  (bases 1 to 592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1221312110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-16- 16"; volume: "110"; issue: "16"; pages: "6488-93"
COMMENT     Sequence Label: pLNCX2-CFP-VGPDFGR-YFP
FEATURES             Location/Qualifiers
     source          1..592
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(55..57,58..60,61..573)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(100..121)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(361..380)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcagatccgc tagcgctacc ggactcagat cagctagcgc taccggtcgc caccatggtg
       61 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      121 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      181 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      241 accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      301 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      361 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      421 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      481 gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc
      541 aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cg
//
