LOCUS       sequence-95859-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9/gRNA Entry expression plasmid .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Gonzalez F, Zhu Z, Shi ZD, Lelli K, Verma N, Li QV, Huangfu D
  TITLE     An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome 
            Editing in Human Pluripotent Stem Cells.
  JOURNAL   Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 
            10.1016/j.stem.2014.05.018.
  PUBMED    24931489
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 
            10.1016/j.stem.2014.05.018."
COMMENT     Sequence Label: piCRg Entry
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..108
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      133..142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             142..207
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             214..234
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atccccccca aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg
       61 atgagcaatg cttttttata atgccaactt tgtacaaaaa agcaggctcc gcggttttca
      121 ggttggaccg gtgccaccat ggactataag gaccacgacg gagactacaa ggatcatgat
      181 attgattaca aagacgatga cgataagatg gccccaaaga agaagcggaa ggtcggtatc
      241 cacggagtcc cagcagccga caagaagtac agcatcggcc tggacatcgg caccaactct
      301 gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg
      361 ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc
      421 ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg
      481 aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac
      541 agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg
      601 caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc
      661 taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat
      721 ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac
//
