LOCUS       sequence-95875-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  raise antibody against Rec8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Chiang T, Duncan FE, Schindler K, Schultz RM, Lampson MA
  TITLE     Evidence that weakened centromere cohesion is a leading cause of 
            age-related aneuploidy in oocytes.
  JOURNAL   Curr Biol. 2010 Sep 14;20(17):1522-8. doi: 
            10.1016/j.cub.2010.06.069.
  PUBMED    20817534
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2010.06"; journalName: "Curr Biol"; date: 
            "2010-09-14- 14"; volume: "20"; issue: "17"; pages: "1522-8"
COMMENT     Sequence Label: His-tagged FL Rec8
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    126..265
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(191..210)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     351..373
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(367..558)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      181 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      241 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      301 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      361 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      421 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      481 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      541 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat
      601 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
      661 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
      721 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag
      781 ccagcagcat cctgcgatgc agatccggaa cataatggtg ca
//
