LOCUS       sequence-95878-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GCaMP6s.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRVdG-4GCaMP6s
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      206..215
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             221..238
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             242..274
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(278..286,287..301)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      308..317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             329..385
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     598..619
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(725..746)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atcccgcaaa tttatcactt gtttacctct ggaggagaga acatatgggc tcaactccaa
       61 cccttgggag caatataaca aaaaacatgt tatggtgcca ttaaaccgct gcatttcatc
      121 aaagtcaagt tgattacctt tacattttga tcctcttgga tgtgaaaaaa actattaaca
      181 tccctcaaaa gaccccggga aagccgccac catgggttct catcatcatc atcatcatgg
      241 tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa
      301 ggatctcgcc accatggtcg actcatcacg tcgtaagtgg aataagacag gtcacgcagt
      361 cagagctata ggtcggctga gctcactcga gaacgtctat atcaaggccg acaagcagaa
      421 gaacggcatc aaggcgaact tccacatccg ccacaacatc gaggacggcg gcgtgcagct
      481 cgcctaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
      541 ccactacctg agcgtgcagt ccaaactttc gaaagacccc aacgagaagc gcgatcacat
      601 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
      661 gggcggtacc ggagggagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      721 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      781 tgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
//
