LOCUS       sequence-95892-e       15023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre-ER knockin targeting construct for mouse endogenous Nanog locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15023)
  AUTHORS   Maza I, Caspi I, Zviran A, Chomsky E, Rais Y, Viukov S, Geula S, 
            Buenrostro JD, Weinberger L, Krupalnik V, Hanna S, Zerbib M, Dutton 
            JR, Greenleaf WJ, Massarwa R, Novershtern N, Hanna JH
  TITLE     Transient acquisition of pluripotency during somatic cell 
            transdifferentiation with iPSC reprogramming factors.
  JOURNAL   Nat Biotechnol. 2015 Jun 22. doi: 10.1038/nbt.3270.
  PUBMED    26098448
REFERENCE   2  (bases 1 to 15023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Jun 22. doi: 10.1038/nbt.3270."
COMMENT     Sequence Label: Nanog-CreER targeting construct
FEATURES             Location/Qualifiers
     source          1..15023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      376..385
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1935..2963
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             2976..3911
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             3024..3917
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     polyA_signal    4227..4348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(4364..4397)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(4981..6006)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(4981..6006)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6025..6520)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6073..6092)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     6462..6485
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(6536..6569)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             join(12171..12239,12240..13031)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12389..12408)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13202..13790
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13691..13710
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    13852..13986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          14047..14113
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(14191..14763)
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
ORIGIN
        1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataatt
      121 aacaatggtg gcttggtggc agtgctgaca gtggtagtat tggtagcatt ggtggcagtg
      181 gaagaaggga agtggtggta atggtggcag gggtggcagc agaggcagac agtggtgacg
      241 atggtggcag tggtgctgtg gcacagtggt ggcattggtg gcatggcaca gtggtggcaa
      301 tgatggtgtg gtgcagtggt agcagtggag acagtggtgg cagtggagac agtagtagta
      361 tggtggctgt ggtggccatg gcggcagtgg tggcacatac aggcctttaa tgccagcaca
      421 tgggaggcag aggcagttgg ctctctgtga gttggaagcc agcttgttct acagagcaag
      481 tcccaggaca gccagggcta cacagagaaa ccctgcctag aataataaca aaactcaaac
      541 aaacaccacc aacccccgcg ctccgccccg ccccccccaa aaaaaacccc aaaccaaaaa
      601 gagccattca agcttagtct tttgtagaat aaaacccagg aagaaccact cctaccaata
      661 ctcactgtgg tagagtcttc acatgggaga tgttacgaga ggacggccct tccctctctg
      721 cttatacaca gaagccgact taagctgggt tagagtgctt tcactcactt atctgtgagc
      781 acaaggactg atcggcaaac tttgaacttg ggatgtggaa ataggaagat caggagtttg
      841 ggaccagcta gagcaataga ctgtgaggcc agtctcgggt acgtgaaact ctatctcaaa
      901 gaaatgacca aaaccaaatt tgaaatacga taaatttctt cttccattgc ttagacggct
      961 gaggcacttg ggattgcagt aagtctgaag ccagcctagg ttttacagtg agaacttgtc
     1021 tcaaaacaaa acagaaggcc tctacattaa tttaaacact ccttaaattg ggcatggtgg
     1081 tagacaagcc tggtctacat actgagtata agctactcaa ggcaacagag aaaaacctgt
     1141 ctcaaaacca aagcatggac caacttacta aggtagcccg agtcttaagc aggacacagg
     1201 ctctttcttc agacttgcgt taaaaagccg cacttttgga gggaagattt ccccaggttt
     1261 cccaatgtga agagcaagca agaaacgctg agtgctgaaa ggaaagccgt gtataaacag
     1321 agactttggc agcaaggtct gactctttca tgtctgtaga aagaatggaa gaggaaactc
     1381 agatccccca cttgacctga aacttcccac tagagatcgc cagggtctgg aggtgcagcc
     1441 gtggttaaaa gatgaataaa gtgaaatgag gtaaagcctc tttttggggg ggggggatgt
     1501 ctttagatca gaggatgccc cctaagcttt ccctccctcc cagtctgggt caccttacag
     1561 cttcttttgc attacaatgt ccatggtgga ccctgcaggt gggattaact gtgaattcac
     1621 agggctggtg gggcgtgggt gccgcctggg tgcctgggag aatagggggt gggtagggta
     1681 ggaggcttga ggggggagga gcaggaccta ccctttaaat ctatcgcctt gagccgttgg
     1741 ccttcagata ggctgatttg gttggtgtct tgctctttct gtgggaaggc tgcggctcac
     1801 ttccttctga cttcttgata attttgcatt agacatttaa ctcttctttc tatgatcttt
     1861 ccttctagac actgagtttt ttggttgttg cctaaaacct tttcagaaat cccttccctc
     1921 gccatcacac tgacatgtcc aatttactga ccgtacacca aaatttgcct gcattaccgg
     1981 tcgatgcaac gagtgatgag gttcgcaaga acctgatgga catgttcagg gatcgccagg
     2041 cgttttctga gcatacctgg aaaatgcttc tgtccgtttg ccggtcgtgg gcggcatggt
     2101 gcaagttgaa taaccggaaa tggtttcccg cagaacctga agatgttcgc gattatcttc
     2161 tatatcttca ggcgcgcggt ctggcagtaa aaactatcca gcaacatttg ggccagctaa
     2221 acatgcttca tcgtcggtcc gggctgccac gaccaagtga cagcaatgct gtttcactgg
     2281 ttatgcggcg gatccgaaaa gaaaacgttg atgccggtga acgtgcaaaa caggctctag
     2341 cgttcgaacg cactgatttc gaccaggttc gttcactcat ggaaaatagc gatcgctgcc
     2401 aggatatacg taatctggca tttctgggga ttgcttataa caccctgtta cgtatagccg
     2461 aaattgccag gatcagggtt aaagatatct cacgtactga cggtgggaga atgttaatcc
     2521 atattggcag aacgaaaacg ctggttagca ccgcaggtgt agagaaggca cttagcctgg
     2581 gggtaactaa actggtcgag cgatggattt ccgtctctgg tgtagctgat gatccgaata
     2641 actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc gccatctgcc accagccagc
     2701 tatcaactcg cgccctggaa gggatttttg aagcaactca tcgattgatt tacggcgcta
     2761 aggatgactc tggtcagaga tacctggcct ggtctggaca cagtgcccgt gtcggagccg
     2821 cgcgagatat ggcccgcgct ggagtttcaa taccggagat catgcaagct ggtggctgga
     2881 ccaatgtaaa tattgtcatg aactatatcc gtaacctgga tagtgaaaca ggggcaatgg
     2941 tgcgcctgct ggaagatggc gatctcgagc cgtctgctgg agacatgaga gctgccaacc
     3001 tttggccaag cccgctcatg atcaaacgct ctaagaagaa cagcctggcc ttgtccctga
     3061 cggccgacca gatggtcagt gccttgttgg atgctgagcc ccccatactc tattccgagt
     3121 atgatcctac cagacccttc agtgaagctt cgatgatggg cttactgacc aacctggcag
     3181 acagggagct ggttcacatg atcaactggg cgaagagggt gccaggcttt gtggatttga
     3241 ccctccatga tcaggtccac cttctagaat gtgcctggct agagatcctg atgattggtc
     3301 tcgtctggcg ctccatggag cacccaggga agctactgtt tgctcctaac ttgctcttgg
     3361 acaggaacca gggaaaatgt gtagagggca tggtggagat cttcgacatg ctgctggcta
     3421 catcatctcg gttccgcatg atgaatctgc agggagagga gtttgtgtgc ctcaaatcta
     3481 ttattttgct taattctgga gtgtacacat ttctgtccag caccctgaag tctctggaag
     3541 agaaggacca tatccaccga gtcctggaca agatcacaga cactttgatc cacctgatgg
     3601 ccaaggcagg cctgaccctg cagcagcagc accagcggct ggcccagctc ctcctcatcc
     3661 tctcccacat caggcacatg agtaacaaag gcatggagca tctgtacagc atgaagtgca
     3721 agaacgtggt gcccctctat gacctgctgc tggagatgct ggacgcccac cgcctacatg
     3781 cgcccactag ccgtggaggg gcatccgtgg aggagacgga ccaaagccac ttggccactg
     3841 cgggctctac ttcatcgcat tccttgcaaa agtattacat cacgggggag gcagagggtt
     3901 tccctgccac ggtctgacag ctcctcctca tcctctccca catcaggcac atgagtaaca
     3961 aaagaatgga gcatctgtac agcatgaagt gcaagaacgt ggtgcccctc tatgacctgc
     4021 tgctggagat gctggacgcc caccgcctac atgcgcccac tagccgtgga ggggcatccg
     4081 tggaggagac ggaccaaagc cacttggcca ctgcgggctc tacttcatcg cattccttgc
     4141 aaaagtatta catcacgggg gaggcagagg gtttccctgc cacagtctga gagctccctg
     4201 gcggaattcg gatcttatta aagcagaact tgtttattgc agcttataat ggttacaaat
     4261 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4321 gtttgtccaa actcatcaat gtatcttatc atgtctggtc gaggaagttc ctatactttc
     4381 tagagaatag gaacttccgc gccgcacaca aaaaccaaca cacagatcat gaaaataaag
     4441 ctcttttatt ggtaccgcag ctgaagcttc tgatggaatt agaacttggc aaaacaatac
     4501 tgagaatgaa gtgtatgtgg aacagaggct gctgatctcg ttcttcaggc tatgaaactg
     4561 acacatttgg aaaccacagt acttagaacc acaaagtggg aatcaagaga aaaacaatga
     4621 tcccacgaga gatctataga tctatagatc atgagtggga ggaatgagct ggcccttaat
     4681 ttggttttgc ttgtttaaat tatgatatcc aactatgaaa cattatcata aagcaatagt
     4741 aaagagcctt cagtaaagag caggcattta tctaatccca ccccaccccc acccccgtag
     4801 ctccaatcct tccattcaaa atgtaggtac tctgttctca cccttcttaa caaagtatga
     4861 cagtaaaaaa cttcatttta gtggacatct ttattgttta atagatcatc aatttctgca
     4921 gaaaaacttc cttttagtgg acatctttat tgtttaatag atcatcaatt tctgcatcga
     4981 ctattccttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc
     5041 tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt
     5101 cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa
     5161 attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg
     5221 gagccgcggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc
     5281 catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc
     5341 gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt
     5401 gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag
     5461 catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg
     5521 ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg
     5581 accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc
     5641 gatcgcatcc atggcctccg cgaccggctg cagaacagcg ggcagttcgg tttcaggcag
     5701 gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa
     5761 ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac
     5821 ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc
     5881 tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga
     5941 gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt
     6001 tttcatggtg gcggctggat cggtcgaaag gcccggagat gaggaagagg agaacagcgc
     6061 ggcagacgtg cgcttttgaa gcgtgcagaa tgccgggctc cggaggacct tcgggcgccc
     6121 gccccgcccc tgagcccgcc cctgagcccg cccccggacc caccccttcc cagcctctga
     6181 gcccagaaag cgaaggagaa agctgctatt ggccgctgcc ccaaaggcct acccgcttcc
     6241 attgctcagc ggtgctgtcc atctgcacga gactagtgag acgtgctact tccatttgtc
     6301 acgtcctgca cgacgcgagc tgcggggcgg gggggaactt cctgactagg ggaggagtag
     6361 aaggtggcgc gaaggggcca ccaaagaacg gagccggttg gcgctaccgg tggatgtgga
     6421 atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg ccagcggggc tgctaaagcg
     6481 catgctccag actgccttgg gaaaagcgcc tcccctaccc ggtagaattg aattcgaagt
     6541 tcctatactt tctagagaat aggaacttcg cggccgcatg agtgtgggtc ttcctggtcc
     6601 ccacagtttg ccctagttct gaggaagcat cgaattctgg gaacgcctca tcaatgcctg
     6661 cagtttttca tcccgagaac tattcttgct tacaagggtc tgctactgag atgctctgca
     6721 cagaggctgg taaggaattc agtccccgaa gaacccagta aattagggta agagtagaaa
     6781 aaacgggctg aagggttatt gcttctttat gaaccgtagt agtcattaac ataagcgggt
     6841 gtccttatca ctcttctgga aacccctctg agtttgaccg gtgatacgtt ggccttctag
     6901 tctgaaatag agatccggga cacaggacgg agcaatataa ggctcttgtg tgtatttgag
     6961 aacctaaata tttgggctct ttggaatatg ttcgggggca gtgagtggtg tcttcagtag
     7021 cagggagacc taacatccta gcttggaatg agcaccccat ttttttggtg aggttataca
     7081 gttagtttgc aatttgccca aatggccgtt ttctccagca gaagcagtta gcatttctct
     7141 caacctttgg gaatatggat gtcacttagc agttctgggt ttgtgagctc agtgctcctt
     7201 ccaaacccca aacaagtgta cacagtttaa gtcattcatt cttatagcta gcatctggag
     7261 ccaaggtctg aagctcattg tctgtacaga gtcagcccag tactcaggct tgtaaatggt
     7321 agaaccaaga ggctgctagg ctgtgtgtgc cacggtgctc aggtgcgtgt tgtgatgctc
     7381 gggtgtgtgc agcgatgctc gggtgtgtgc agtgatgctc gggtgtgtgc agcggtgctt
     7441 gggtgtgtgg agcgctagga agtgtgtgtc cccttttttc tgagctttta agttaatgtc
     7501 tgtcatcttg atttttctga tttatgggtt caatttttga tttttttttt gtttgttttt
     7561 ttttgttttt tcgagacagg gtttctctgt gtagccctgg ctgtcctgga actcactttg
     7621 tagaccaggc tggcctcaaa ctcagaaatc cgcctgcctc tgcctctgcc tccctcaatt
     7681 tttgattttg gaggtgggaa aagtgctggg ttaggaatga acgggtgggg atagcctttg
     7741 taagtgatgt gtgtttttag agcatgtgtg tagagcatgt gtgtttgtag tgcgtgcccc
     7801 tagtgccagc agatggcata attttccatt tagaagctgc tttcagggct gtctacttat
     7861 ttgctagact gcaggctaac ctcagcattg ccatcagcgt ggagcacaga tgtcttcagt
     7921 gtaggaggtg tcttctacct gctgctggat ctaggaaatg ttggggcaaa gcttggtgga
     7981 ggagcacttg tctcttgctg aaggccctgg aaaaaaacaa aaaagctaac cgaagagcta
     8041 gagggtcaag gataagagaa acgaatttgg ttaaccgaaa ttttactcta gtaattaata
     8101 attaactgga ccctctgact ggctgctctt gtactagcag aaaagatggt gggatatttt
     8161 tatttattta tttttttaaa gatttattta ttatatgtaa gtacactgta gttgtcttca
     8221 gacactccag aagagggcgt cagatcttgt tacagatggt tgtgagccac catatggttg
     8281 ctgggatttg aactccagac cttcggaaga gcagttgggt gctcttaccc actgagccgt
     8341 tggatgctct tacccactga gccatctcac cagccccagt gggatcattt taaaatatac
     8401 ctttaaaaaa aaattggggg tggggggtgt ctaaactcag gactgtgcat tcctaacaac
     8461 aagtgattta accctggaaa tttccttaga taacattcag tataatggat ttcgtcatga
     8521 cattttttgt tcataaatat tagttcatcc ctaaatgtta atgtgtgtgt gtgtgtgtgt
     8581 gtgtgtatgt atgtatgtat gtatgtatgt atatatgtat gtatgtatag atatagctga
     8641 ctcagtggta gtagagtatg aaaggtccca acaggattct attcacccag caccatgcaa
     8701 aaatctaggc gagataaaag tccccccaaa cctgagtctt tggataaacc tccactcaga
     8761 aggtagagag gtaggaccca gagagcaggc cagccttggc tacctgagac cctatccctt
     8821 agaaagaaaa gaaaaccaca aacatagact agatcctctg ctgcctaagc tcttgtgctg
     8881 tcttgtctgt ctgtctctat ttgttgagac gggctgatct caaattctcc catctcagct
     8941 actggagcag gggggcttta gatttgtgct acccactgtc aggatcagag gtttacagtg
     9001 tagatgcggt cacggttcta atgaagaggg agcagggaga agggattgga aagagcagac
     9061 tacaaactca aacctgtctt catcctgcca ttgaactgag tattgtttgg ttagttgtct
     9121 tttttttttt ttaaatagga ttcccagggt ctaattgtat aatttaaata actaatgaaa
     9181 cttaaaaaca ctgtatgtag ggctggtgag atggctcaac aggtaaaagc actgactgcc
     9241 cttcccaaag tcctgagttc aaatcccagc aaccacatgg tggctcacaa ccatccgtaa
     9301 tgagatctga tgccctcttc tggtgtgtct gaagacagct acagtgtact tacatataat
     9361 aataaataaa tatttagcag agacattctt tttttttttt aattaattaa tcccatgtgg
     9421 ttgctgggaa ttgaactcag gaccttcgga agagcagtct gcactcttaa ccttaaccgc
     9481 tgagccatct ctccagcccc ctaagtagaa acattcttaa aaaaaaaaaa aaaaaaaaaa
     9541 aaaacactat aaaagggaga tctatgcaaa atggtgttac ccaggggtga caaagtattc
     9601 caagaatact ttccaaaaat taggtttttc ttgttactta gatctatgaa gataattgct
     9661 ttgaaagtaa atatagccgg gcgtttaatc ccagcacttg ggaggcagag gcaggcgcat
     9721 ttctgagttc gaggccagcc tggtctacaa agtgagctcc aggacagcca gggctataca
     9781 gagaaaccct gtctcgaaaa aaccaaaaaa aaagaaaaaa aaaaaaaaaa aagaaagaaa
     9841 gaaagaaaga aagaaagaaa gaaagaaaga aagaaagtaa gtaaatatat taaatgtaac
     9901 atttaattat ctgggaaaat gatttatttg tttggttggt tttgtttttg tttttttggt
     9961 cagggtttct ctgtgtagcc ctggccatcc tggaatttac tttgtaaaac tgactggcct
    10021 ttttaaaatt tatttattat atgtaagtac actgtagctg tcttcagaca caccagaaga
    10081 gggcatcaga tctcattacg gatggttgtg agccaccatg tggttgctgg gatttgaact
    10141 caggaccttc ggaagagcag tcagtgctct taaccgctga gccataaccc ccaccccacc
    10201 ccgtgaactg actggactta aacccataat ctgcctgctt gagtgtccca agtgctggga
    10261 ctaaaggtat gtaccaccat gccaggctga gaatgtctta tttattaaca agttatctat
    10321 ttatttctaa cagcctctcc tcgcccttcc tctgaagacc tgcctcttca aggcagccct
    10381 gattcttcta ccagtcccaa acaaaagctc tcaagtcctg aggctgacaa gggccctgag
    10441 gaggaggaga acaaggtcct tgccaggaag cagaagatgc ggactgtgtt ctctcaggcc
    10501 cagctgtgtg cactcaagga caggtttcag aagcagaagt acctcagcct ccagcagatg
    10561 caagaactct cctccattct gaacctgagc tataagcagg tggggtcctt agaagttgct
    10621 gtaatgtaac acaatgagac tgacttgcta atgaatcgaa ctaacgtctg gacgtctctc
    10681 taactatcct atcctcctat cctatgcgcc tgttaaaatc ataattatgt gattgattga
    10741 ttgattgatt atgtataagt acactatagc tgtcttcaga tacaccagaa gagggcatca
    10801 gatctcatta cggatggttg tgagccacca tgtggttgct gggatttgaa ctcaggacct
    10861 tcggaagagc agtcagtgct cttaaccgct gagccatctc tccagcccca attatgtgat
    10921 ttatatattt cccagtttgt ttcattggtt actttatctt ctacttcacc tcccacaccc
    10981 atccccaaat cagggtctca ctgtagccct gactggtctc tgtagaccag gctgggagag
    11041 gttcacctgc ctctgtctcc tgggtgctgg aaataaaggt gtgcctggct tttttccttt
    11101 cttttctttt ttaagattgt ttatttttaa attcctttag ttcattggtg ttttgcctgc
    11161 atgtgtttgt ctttgtgaga gtatcagatc tcctggacta gagttacaga cagtcgtgag
    11221 ctgccatgtg gccgctgaga attgaaccca ggtcctctgg aagagcagcc agtgctctta
    11281 acagaagagc catttccccc agcacctgtc tttttcaaga aagagtttca ctttacaggc
    11341 ctggctgtca tgtttaaggt cgggctgtag acctgtctgc attctgtatg tcttagcttg
    11401 ttcgaatgtc ctgtcactct gcaggttaag acctggtttc aaaaccaaag gatgaagtgc
    11461 aagcggtggc agaaaaacca gtggttgaag actagcaatg gtctgattca ggtaacaaga
    11521 tgttttctct gctttccttt ttccccctcc atcttaccat cttattttat gtgtctgtgc
    11581 cgtgtgtgtg tgtgtgtgtg tgtgtgtggc tgacctatgg ctgttgggta tggatcccag
    11641 atttcctgta agagcagtca gtggctctgc ctttgcttcc cagtgtcatc ctgatccatc
    11701 tatgtccatt agtcctgtcc ctttcccatc cctcttcctc cacgcactct ttcttgtgat
    11761 ctttggttct ttgaggcagg ctctctctat gtagccctgg cagtcagaga tctgcctgcc
    11821 tagtctcagg agtgctgggg ttaacggcct gtgcggccat gctaatcttt tcagtatttc
    11881 tgcagtttac atctttagag tccccttttc actcattgca ttaatcacct ctttcagaag
    11941 ggctcagcac cagtggagta tcccagcatc cattgcagct atccccaggg ctatctggtg
    12001 aacgcatctg gaagcctttc catgtggggc agccagactt ggaccaaccc aacttggagc
    12061 agccagacct ggaccaaccc aacttggaac aaccagacct ggaccaaccc aacttggagc
    12121 agccaggcct ggaccgctca gtccgtcgac tcgattgaaa aaggaagagt atgagtattc
    12181 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    12241 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    12301 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    12361 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
    12421 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    12481 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    12541 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    12601 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    12661 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    12721 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    12781 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    12841 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    12901 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    12961 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    13021 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    13081 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    13141 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    13201 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    13261 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    13321 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
    13381 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    13441 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    13501 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    13561 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
    13621 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    13681 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    13741 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    13801 acgcggcctt tttacggttc ctggcctttt gccggtccgc tctagaacta cgatccagac
    13861 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
    13921 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
    13981 caagttagat cctagtggat ctgcattcca ccactgctcc cattcatcag ttccataggt
    14041 tggaatctaa aatacacaaa caattaggaa tcagtagttt aacacattat acacttaaaa
    14101 attttatatt taccttagag ctttaaatct ctgtaggtag tttgtccaat tatgtcacac
    14161 cacagaagta aggtttcctt cacaaagaga tcgcctgaca cgatttcctg cacaggcttg
    14221 agccatatac tcatacatcg catcttggcc acgttttcca cgggtttcaa aattaatctc
    14281 aagttctacg cttaacgctt tcgcctgttc ccagttatta atatattcaa cgctagaact
    14341 cccctcagcg aagggaaggc tgagcactac acgcgaagca ccatcaccga accttttgat
    14401 aaactcttcc gttccgactt gctccatcaa cggttcagtg agacttaaac ctaactcttt
    14461 cttaatagtt tcggcattat ccacttttag tgcgagaacc ttcgtcagtc ctggatacgt
    14521 cactttgacc acgcctccag cttttccaga gagcgggttt tcattatcta cagagtatcc
    14581 cgcagcgtcg tatttattgt cggtactata aaaccctttc caatcatcgt cataatttcc
    14641 ttgtgtacca gattttggct tttgtatacc tttttgaatg gaatctacat aaccaggttt
    14701 agtcccgtgg tacgaagaaa agttttccat cacaaaagat ttagaagaat caacaacatc
    14761 atcagggtcc atggcgagga cctgcaggtc gcagatcctg aacggcagag gttacggcag
    14821 tttgtctctc ccccttccgg gagccacctt cttctccaac cgtcccggtc gcgctctcgg
    14881 cgcttctgag gagagaactg gctgagtgac gccctttata gattcgccct tgtgtcccgc
    14941 ccttcctttc ccgccctccc ttgcgctacg gggccgcccg caccggccta cacggagcgc
    15001 gcgcggcgga gttgttcggt ccg
//
