LOCUS       sequence-95900-e        4449 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged mouse TUT7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Liu X, Zheng Q, Vrettos N, Maragkakis M, Alexiou P, Gregory BD, 
            Mourelatos Z
  TITLE     A MicroRNA Precursor Surveillance System in Quality Control of 
            MicroRNA Synthesis.
  JOURNAL   Mol Cell. 2014 Aug 27. pii: S1097-2765(14)00610-8. doi: 
            10.1016/j.molcel.2014.07.017.
  PUBMED    25175028
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2014 Aug 27. pii: S1097-2765(14)00610-8. doi: 
            10.1016/j.molcel.2014.07.017."
COMMENT     Sequence Label: pcDNA3-FLAG-mTUT7
FEATURES             Location/Qualifiers
     source          1..4449
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggactaca aagacgatga cgacaaggga gatacagcaa aaccttactt tgtgaagcgc
       61 actaaagacc gtggaattat tgatgatgat gatttcagaa ggggccatcc ccaacaagat
      121 tatttaataa tggatgatta tgctaaaggc catagcagta aaatggaaaa gggccttcca
      181 aaaaagaaga tatcacccgg gaactatggg aacaccccca ggaaaggact gtatggggtt
      241 tccagcaatc catatgcatt taaaaaccca atttacagtc agcctgcttg gatgaatgat
      301 aaccacaaag atcaaaataa aaaatggcta tctgatgaac tcgctggtaa tgcagacagc
      361 tggagagagt tcaagcccgg acctaggatt cctgtcataa gccgctcaag gaaggaatcc
      421 tttcaggaaa gtgacgatgc ctacaggtgg caggagggga gaggctgcag agctgtgaga
      481 aggctgtttc agaaagacct cagcagccta gaagccatgt cagaaatgga agcaggaagt
      541 cctgaaaaca agaaacagcg gtccagacct cgtaagccac ggaggactag aacggaggac
      601 agtgagcagg atggagatct ggacggtcct gtcatcgacg agtctgtcct ttcaaccaag
      661 gagctgttgg gcctccagca ggctgaggaa cggctcaaga gagactgcat cgacaggctg
      721 aaaaggcgac cacgaaactg cccgacagca aagtatacct gcaaactgtg cgatgctttg
      781 attgactcca ttccgtttgc tcataagcac atcaaggaga agaggcacaa gaagaacctt
      841 aaggaaaagc aggaggaaga attgctcacc acactgcccc cgccagcgcc ctcccagata
      901 catgcagttg gcagcgccat tgacagagtg gtgcaggagt ttggtttaca cagtgaaaac
      961 ttggatcaga gactagaaat taagcgtgtc atggaaagtg tgttccggca caagctacca
     1021 gactgttctc ttaggcttta tggatcctcc tgtagccgac tgggcttcag agactctgac
     1081 gtgaacattg atgttcagtt tccagctgtc atgtctcagc cagatgtcct cttacttgtt
     1141 caagaatgtt taaagaacag tgactccttt attgatgttg atgctgattt ccatgcccgg
     1201 gtccctgtgg tggtgtgcag agataagcaa agcggccttc tttgtaaagt aagtgcagga
     1261 aacgaaaacg cttggctgac cacaaagcac ctaacagctc ttggaaaact agagccaagg
     1321 ctggttcctc tggtgattgc attcagatac tgggcaaagc tttgtagtat tgatcgcccc
     1381 gaagaaggag gcctgccacc atatgtgttt gctctgatgg ctgttttctt ccttcagcaa
     1441 agaaaagaac ctctcttgcc agtttatcta ggatcatgga ttgaagaatt ctcattaaat
     1501 aaattaggga atttcagcct taaagatgtt gagaaagact ctgtggtctg ggaatacact
     1561 gacaacagca caggggacac cagctcagca aaagaggagg cacccaaaga gactgcagca
     1621 aagaagggac aggtgccatt gacatttaat ataaagcatc agccttcagt gccagtcggg
     1681 caactctggg tggaattgct gcggttctat gctttagagt ttaatctggc tgatttagtg
     1741 atcagtattc gtgtcaaaga attaatatca cgggaatcaa aggactggcc caaaaagcgt
     1801 atcgcaattg aagaccccta ttctgttaaa agaaatgtgg caaggaccct gaataaccag
     1861 cctgtgtttg agtatatact ccattgtttg aggacaacat ataaatattt cgctcttcca
     1921 cacaaagtta caaaacccaa cctcacaaag cctcctagcc cagtcacctg tgtttcagac
     1981 ccttacagag aagcaaaaaa tggtggccca gaaccccaag caacaaatat tgataaactg
     2041 ggaaatgcag ctgtggctca agatcctggt gttcaaacct caggtgactg cagagcacag
     2101 ctagttactc ttaagaatac cactgaggag gtgggaagcc cagcaaagga gaagacagga
     2161 ggtgtgcaca tcccagccca ccaggagagc tctggctgtg tccaggcaga ggtcagttgt
     2221 gagggtttgg aggatgccac agcagagctt ccggaaacag gaagtgacaa cgaggaagtc
     2281 agaaggaaga ccaagcatcc tttgagcact gatgaccaag gtttaagcag cagcaagcac
     2341 ccagaactgc agaactgtgg cagcctgtgt ggtctgcagg cagacaacac tctggagtta
     2401 gtagctgaag agtgtaatag ctgtgcctct ttggacaaca aggctgaggt caatgaagag
     2461 aggatagaag gcgctgagga actagaagaa gcagcagctc tgagttgctt ttccccttca
     2521 gtacagagtc gcacatctgc agccatgcac tttgatgatg aggaggagga ggaggaagaa
     2581 gaggaggaag aggagcccag gctcagcatt aaccttacag aagatgagga gggggttgct
     2641 aatgagcatc aggtggacag caggtatgct gggtcaggtg aggaggatgc cctctctgaa
     2701 gaagatgact tggcagagcc tgctaagggt gaagatacag gagaatgtgg agaaaacgtg
     2761 ggtgggactc tgctcataga cctaaataga ataacactta aagaagaaag ctttcctgag
     2821 gaagatttac ctggggacca gtctgagttc ttctacgaat ttagaaaact gaccttcacc
     2881 aaaggcaagt cccccacagt agtgtgcagc ttgtgtaagc gagaaggtca cctaaagaag
     2941 gactgtcctg aggacttcaa gagggtccag ctggagccgt tgccacccct gacacccaag
     3001 ttctcaaaca tcttagatca agtctgcgtc cagtgttaca aggatttttc tccaactatt
     3061 gtagaagatc aggctcgtga acatatccgg caaaatctag aaagtttcat aaaacaggat
     3121 tttccaggaa ctaaattgag cttgtttggc tcctccaaaa atggatttgg gttcaaacag
     3181 agtgaccttg acgtctgtat gacgattaac ggacatgaga ctgctgaggg gctggactgt
     3241 gtaagaacta ttgaggagct ggctcgagtc ctcagaaagc actcagggtt gaggaatatc
     3301 cttcctatca caacagccaa ggtgcccatc gtgaagttct tccatctaag aagtggtctg
     3361 gaagtggaca tcagcttgta taacacactg gcgcttcata acacaaggct cttgtctgca
     3421 tactcagcca ttgaccccag agtgaagtat ctgtgctaca ccatgaaagt gttcacaaag
     3481 atgtgtgaca tcggtgacgc gtccagaggc agcttgtcat cctacgctta cactcttatg
     3541 gtgctatact tcctccagca aaggtctcca cctgtcatcc ccgtgcttca agagatatac
     3601 aaaggtgaaa agaaaccaga aatacttgtt gatggctgga atatttactt tttcgatcaa
     3661 ataaatgaac tgcccacttg ttggccagaa tatgggaaaa atacagaacc tgtcgggcag
     3721 ctgtggctgg gacttctgcg cttctacact gaggagtttg actttaagga gcacgtgatc
     3781 agcatcagaa gaaagagtct gcttacaact tttaagaaac agtggacctc aaaatacatt
     3841 gttattgaag atcccttcga tttgaatcac aatcttggtg ctggattatc gaggaaaatg
     3901 acaaatttta taatgaaagc ttttatcaat ggtagaagag tatttggaat tccagtcaaa
     3961 ggatttccaa aggacaaccc ctcaaaactg gcatactttt ttgatccgga tgtcctaact
     4021 gagggagaac tggccctaaa tgacaggtgc tgccgaattt gtgggaaaat cgggcacttc
     4081 atgaaggact gtcccatgag gaggaaagtg aggcgacgga gagaccagga agatacccca
     4141 aaccaaaggt actctgagag caaagaaaaa agaagcaaag aggacaaaga gattcagaac
     4201 aagtacacag aaaaggaagt gtcaacaaaa gaagataagc tcacaccgtg tgcagctgcg
     4261 aaagccaagc cagtgagggc agctgtcgac ctgggccggg agaagctcct caggacgccc
     4321 acagaaaagt ggaagagaca ggacgacaga gactcgagag aaaagcgttg tttcatctgt
     4381 ggaagagaag gacacattaa aaaggaatgc ccacagttca aaggctctcc aggcagtgct
     4441 tttacatga
//
