LOCUS       sequence-95902-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Liou J, Kim ML, Heo WD, Jones JT, Myers JW, Ferrell JE, Meyer T
  TITLE     STIM is a Ca2+ sensor essential for Ca2+-store-depletion-triggered 
            Ca2+ influx.
  JOURNAL   Curr Biol. 2005 Jul 12. 15(13):1235-41.
  PUBMED    16005298
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2005 Jul 12. 15(13):1235-41."
COMMENT     Sequence Label: pEX-SP-CFP-STIM1(23-685)
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      119..128
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(125..127,128..130,131..841)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(170..191)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(431..450)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     778..799
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    851..875
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcacc atggatgtat gcgtccgtct
       61 tgccctgtgg ctcctctggg gactcctcct gcaccagggc cagagcctcg caccggtcgc
      121 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      181 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      241 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      301 caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat
      361 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      421 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      481 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      541 gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa
      601 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
      661 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
      721 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
      781 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
      841 gtccggatca acaagtttgt acaaaaaagc aggctcc
//
