LOCUS       sequence-95907-e         410 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 410)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-CAG-FLEX-EGFP
FEATURES             Location/Qualifiers
     source          1..410
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     158..180
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     242..261
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(301..334)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
       61 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
      121 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
      181 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
      241 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcga tgactccgga
      301 ataacttcgt ataggatact ttatacgaag ttatgcagaa tggtagctgg attgtagctg
      361 ctattagcaa tatgaaacct cttaataact tcgtatagca tacattatac
//
