LOCUS       sequence-95913-e         469 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to 
            accept gRNA from pUC gRNA Shuttle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA
  TITLE     Targeted genome modifications in soybean with CRISPR/Cas9.
  JOURNAL   BMC Biotechnol. 2015 Mar 12;15:16. doi: 10.1186/s12896-015-0131-2.
  PUBMED    25879861
REFERENCE   2  (bases 1 to 469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2015-03-12"; volume: "15:16. doi"; pages: " 
            10.1186/s12896-015-0131-2"
COMMENT     Sequence Label: p201B Cas9
FEATURES             Location/Qualifiers
     source          1..469
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(276..296)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
       61 ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
      121 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
      181 aaactttatt gccaaatgtt tgaacgatcg gggaaattcg agctcggccg gcctcgagac
      241 gtcgacgcgt aagcttctgc agatatccgc ggtcacacct tcctcttctt cttggggtca
      301 gccctgctgt ctccaccgag ctgagagagg tcgattcttg tttcatagag ccccgtaatt
      361 gactgatgaa tcagtgtggc gtccaggacc tcctttgtag aggtgtaccg ctttctgtct
      421 atggtggtgt cgaagtactt gaaggctgca ggcgcgccca agttggtca
//
