LOCUS       sequence-95920-e         476 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses splitTVA-P2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 476)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-CAG-FLEX-splitTVA-EGFP
FEATURES             Location/Qualifiers
     source          1..476
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..41
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    92..125
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(179..200)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggttaatata acttcgtata atgtatgcta tacgaagtta tgcagaatgg tagctggatt
       61 gtagctgcta ttagcaatat gaaacctctt aataacttcg tataaagtat cctatacgaa
      121 gttatggcgc gccttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca
      181 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact
      241 gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct
      301 gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt
      361 tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt
      421 tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttc
//
