LOCUS       sequence-95927-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: C2CD2_pCSdest
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatgcggct ctcccctttc catttacagc
      181 tggagttcca catgaaggag aagagagagg acctccagat tagctggtct ttcatcagtg
      241 tgccggaaat ggccgttaat atccagccca aagcactggg ggaggaccag gtggctgaga
      301 caagtgcgat gtctgacgtt ctcaaggaca tcttgaagca tttggctggt tctgcctctc
      361 catcagtggt tctgattacc aagcccacga ctgtaaagga agctcagaac ttacagtgtg
      421 ctgcatctac tgctcaggaa tcctgtcctc ctaaacctcc aagggctcac gagctgaagc
      481 tactggtgag gaacatccac gtcttgctgc tcagcgagcc cggtgcctca ggccacatta
      541 atgcagtgtg cgtcgtgcag ctgaacgatc ctgttcagag gttctccagc accctgacga
      601 aaaacactcc ggacctcatg tgggaagagg aattcacctt cgagttgaat gccaagtcga
      661 aggagttaca cctgcagatt tcagaggctg ggcgatcctc agaaggtctg ctggcgacgg
      721 cgacagttcc tctggactta tttaagaagc agccttctgg gccacagagc ttcacgctga
      781 ccagcgggtc tgcctgcggc agctcagtgc tgggctcggt cacggcagag
//
