LOCUS       sequence-95931-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: RRP1_pCSdest
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        55..73
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     55..72
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    127..151
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttaagggaac cgccattctg cctggggacg tcggagcaag cttgatttag
       61 gtgacactat agaatacaag ctacttgttc tttttgcagg atcccatcga ttcgaattca
      121 aggatcacaa gtttgtacaa aaaagcaggc accatggttt cgcgcgtgca gctcccgcct
      181 gagatccagc tggctcagcg cctggcgggg aatgagcagg tgacccggga ccgggcggtg
      241 aggaagctcc ggaaatacat cgtcgccagg actcagcggg ccgcaggtgg ttttacgcac
      301 gacgagctgc tgaaggtgtg gaaaggactg ttttattgca tgtggatgca ggacaagcca
      361 ctcctccagg aagaattagg aaggactatt tcccagctcg ttcatgcttt tcagaccacg
      421 gaggcgcagc acctgttcct tcaggccttc tggcagacca tgaatcgcga gtggacgggc
      481 attgacaggc tgcgcctgga taaattctac atgctcatgc ggatggtcct gaacgagtcc
      541 ttgaaggttc tgaagatgca aggctgggaa gaaagacaga tcgaggagct gctagagctg
      601 ctgatgactg agatcctgca ccccagcagc caggccccca acggtgtgaa gagccacttc
      661 atcgagatct tcctggagga gctgaccaaa gtgggcgccg aggagcttac ggcagaccag
      721 aacctgaagt tcatcgaccc cttctgcaga atcgctgccc ggaccaagga ttccttg
//
