LOCUS       sequence-95933-e         489 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses splitTVA950.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 489)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-CAG-FLEX-splitTVA950
FEATURES             Location/Qualifiers
     source          1..489
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    45..78
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    129..162
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taccagtcaa tctttcacaa attttgtaat ccagaggttg attcataact tcgtataaag
       61 tatcctatac gaagttattc tttgcaccat tctaaagaat aacagtgata atttctgggt
      121 taaggcaaat aacttcgtat aatgtatgct atacgaagtt attaggtgcg cggctgctgc
      181 ccgcgctgct gctgctgctg ctgcccggta acgtgaccgg taacgggtcc ggtaacggtt
      241 ctttgtcccg ttgccccccc ggtcagttcc gctgctcgga gccgcccggt gcccacgggg
      301 agtgttaccc gcaggactgg ctgtgcgacg gacaccccga ctgcgacgac gggcgggacg
      361 agtggggctg cgggaccagc gcgatccccg cggtgcccac ggacaacggc acagaggctc
      421 ccactgcccc tgctcctgga cgtgctctgc cagccaggaa tcacggccgc atgtggatgc
      481 tgatcactg
//
