LOCUS       sequence-95936-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: TRPM2_pCSdest
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        55..73
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     55..72
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    127..151
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttaagggaac cgccattctg cctggggacg tcggagcaag cttgatttag
       61 gtgacactat agaatacaag ctacttgttc tttttgcagg atcccatcga ttcgaattca
      121 aggatcacaa gtttgtacaa aaaagcaggc tccgcggccg cccccttcac catggagccc
      181 tcagccctga ggaaagctgg ctcggagcag gaggagggct ttgaggggct gcccagaagg
      241 gtcactgacc tggggatggt ctccaatctc cggcgcagca acagcagcct cttcaagagc
      301 tggaggctac agtgcccctt cggcaacaat gacaagcaag aaagcctcag ttcgtggatt
      361 cctgaaaaca tcaagaagaa agaatgcgtg tattttgtgg aaagttccaa actgtctgat
      421 gctgggaagg tggtgtgtca gtgtggctac acgcatgagc agcacttgga ggaggctacc
      481 aagccccaca ccttccaggg cacacagtgg gacccaaaga aacatgtcca ggagatgcca
      541 accgatgcct ttggcgacat cgtcttcacg ggcctgagcc agaaggtgaa aaagtacgtc
      601 cgagtctccc aggacacgcc ctccagcgtg atctaccacc tcatgaccca gcactggggg
      661 ctggacgtcc ccaatctctt gatctcggtg accggggggg ccaagaactt caacatgaag
      721 ccgcggctga agagcatttt ccgcagaggc ctggtcaagg tggctcagac cacaggggcc
      781 tggatcatca caggggggtc ccacaccggc gtcatgaagc aggtaggcga ggcggtgcgg
      841 gacttcagcc tgagcagcag ctac
//
