LOCUS       sequence-95937-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: POFUT2_pCSdest
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        55..73
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     55..72
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    127..151
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttaagggaac cgccattctg cctggggacg tcggagcaag cttgatttag
       61 gtgacactat agaatacaag ctacttgttc tttttgcagg atcccatcga ttcgaattca
      121 aggatcacaa gtttgtacaa aaaagcaggc accatggcga cactcagctt cgtcttcctg
      181 ctgctggggg cagtgtcctg gcctccggct tctgcctccg gccaggagtt ctggcccgga
      241 caatcggcgg ccgatattct gtcgggggcg gcttcccgca gacggtatct tctgtatgac
      301 gtcaaccccc cggaaggctt caacctgcgc agggatgtct atatccgaat cgcctctctc
      361 ctgaagactc tgctgaagac ggaggagtgg gtgcttgtcc tgcctccatg gggccgcctc
      421 tatcactggc agagtcctga catccaccag gtccggattc cctggtctga gttttttgat
      481 cttccaagtc tcaataaaaa catccccgtc atcgagtatg agcagttcat cgcagaatct
      541 ggtgggccct ttattgacca ggtttacgtc ctgcaaagtt acgcagaggg gtggaaagaa
      601 gggacctggg aagagaaggt ggacgagcgg ccgtgtattg atcagctcct gtactcccag
      661 gacaagcacg agtactacag aggatggttt tggggttatg aggagaccag gggtctaaac
      721 gtctcctgtc tgtccgtcca gggctcagcc tccatcgtgg cgcccctgct gctgagaaac
      781 acatcagccc ggtccgtgat gttagacaga gccgagaacc tacttcacga ccactatgga
//
