LOCUS       sequence-95941-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: C21orf2_pCSdest
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatgaagct gacgcggaag atggttctga
      181 cccgggccaa ggcctcggag ctgcacagcg tgcgcaagct caactgctgg ggcagccgcc
      241 tcacagatat ctccatttgc caggagatgc ccagcctgga ggtgatcacg ctcagtgtca
      301 acagcatctc caccctggag cctgtgagcc ggtgccagcg cctgagtgag ctgtacctgc
      361 ggaggaaccg catccccagc ctggctgagc tcttctacct gaaggggctg ccgcgtctgc
      421 gggtgctgtg gctggccgag aacccgtgct gcggcaccag cccccaccgc taccgcatga
      481 ccgtgctgcg caccctgccg cgcctacaga agctggacaa ccaggctgtg acggaggagg
      541 agctgtcccg tgcactgagt gagggagagg agatcactgc ggccccagag agagagggca
      601 caggccacgg cggccccaag ctatgctgca cactgagctc cctcagctcc gctgctgaga
      661 ctggccggga cccgctggac agcgaggagg aggcaaccgg cgcccaggat gaacgtggcc
      721 tgaagccgcc ttcccggggc cagtttcctt ccctctcagc cagggatgcc tcgagcagcc
      781 acaggggcag ggtgagtggc gggccgctag gggccgcggc tgcctctgcc cactgca
//
